CAJ | 학술논문

利用正置双光子显微镜系统和荧光探针标记技术，观察脑内Ca2＋分布，建立测量活体动物脑内Ca2＋动态变化的实验方法。制作活体动物颅骨开窗样本，脑内负载Ca2＋标记物Oregon Green 488 BAPTA-1和星型胶质细胞标记物Sulforhodamine 101，利用双光子显微镜分别检测神经元和星型胶质细胞内Ca2＋分布和动作电位引起的Ca2＋瞬变。结果显示双光子显微镜可探测到脑内250μm处荧光信号，图像清晰且信噪比高，并能实时检测神经元和星型胶质细胞内Ca2＋信号的动态变化。活体脑内Ca2＋检测技术平台的建立为基础研究和医药应用提供了在体实验依据。
이용정치쌍광자현미경계통화형광탐침표기기술，관찰뇌내Ca2＋분포，건립측량활체동물뇌내Ca2＋동태변화적실험방법。제작활체동물로골개창양본，뇌내부재Ca2＋표기물Oregon Green 488 BAPTA-1화성형효질세포표기물Sulforhodamine 101，이용쌍광자현미경분별검측신경원화성형효질세포내Ca2＋분포화동작전위인기적Ca2＋순변。결과현시쌍광자현미경가탐측도뇌내250μm처형광신호，도상청석차신조비고，병능실시검측신경원화성형효질세포내Ca2＋신호적동태변화。활체뇌내Ca2＋검측기술평태적건립위기출연구화의약응용제공료재체실험의거。
Using two-photon laser scanning microscopy and fluorescent probe labeling technique,we established a new method for observing the distribution and variation of Ca2+signaling in the mouse brain in vivo.Cranial window surgery of anesthesia mouse was made,Ca2+marker Oregon Green 488 BAPTA-1 and astrocyte marker Sulforhodamine 101 were loaded into the brain,and then Ca2+distribution and Ca2+transients were detected via two-photon microscopy.The re-sults showed that fluorescent signals were able to be detected clearly in the brain at a depth of up to 250 μm with high signal to noise ratio.Ca2+transients was observed in both neurons and as astrocytes.It indicates that the platform for de-tecting Ca2+signaling in vivo by two-photon microscopy has been successfully established,and this platform may provide valuable information for basic research and medical applications.