CAJ | 학술논문

目的：利用正丁醇溶剂提取地榆中的鞣质（15.36％），研究其萃取层对人肝癌细胞株HepG2增殖和凋亡的影响及其机制。方法用不同浓度的正丁醇萃取层作用于人肝癌HepG2细胞，通过MTT法检测细胞生长抑制率，流式细胞术检测肿瘤细胞凋亡率和细胞内活性氧（ROS）的含量。结果 HepG2细胞经不同浓度（100,150,200,250,300μg·mL-1）的地榆正丁醇萃取层作用48 h，细胞增殖明显受到抑制，其IC50为222.87μg·mL-1。不同浓度（200,400,600μg·mL-1）的地榆正丁醇萃取层作用于HepG2细胞48 h后，细胞的凋亡率和细胞内ROS的含量均明显高于空白组（P<0.05），且随着地榆正丁醇萃取层浓度的增加，HepG2细胞的凋亡率和细胞内ROS的含量也逐渐升高。结论地榆正丁醇萃取层可以浓度依赖性方式抑制人肝癌细胞株HepG2的增殖并促进其凋亡，这可能与其促进细胞内ROS的产生有关。
목적：이용정정순용제제취지유중적유질（15.36％），연구기췌취층대인간암세포주HepG2증식화조망적영향급기궤제。방법용불동농도적정정순췌취층작용우인간암HepG2세포，통과MTT법검측세포생장억제솔，류식세포술검측종류세포조망솔화세포내활성양（ROS）적함량。결과 HepG2세포경불동농도（100,150,200,250,300μg·mL-1）적지유정정순췌취층작용48 h，세포증식명현수도억제，기IC50위222.87μg·mL-1。불동농도（200,400,600μg·mL-1）적지유정정순췌취층작용우HepG2세포48 h후，세포적조망솔화세포내ROS적함량균명현고우공백조（P<0.05），차수착지유정정순췌취층농도적증가，HepG2세포적조망솔화세포내ROS적함량야축점승고。결론지유정정순췌취층가이농도의뢰성방식억제인간암세포주HepG2적증식병촉진기조망，저가능여기촉진세포내ROS적산생유관。
Objective To study the effects and mechanisms of n-butanol solvent of extract tannin (15.36%) from the San-guisorba officinalis L. on the proliferation and apoptosis of human liver cancer HepG2 cells. Methods HepG2 cells were treated with different concentrations of n-butanol extract. The growth inhibition ratio of HepG2 cells was measured by MTT. The cell apoptotic rate and intracellular ROS were detected by flow cytometry (FCM). Results After being treated by different concentra-tions of n-butanol extract from Sanguisorba officinalis L. (100, 150, 200, 250, 300μg·mL-1) for 48 h, the proliferation of HepG2 cells was inhibited obviously, and the IC50 was 222.87μg·mL-1. Moreover, with the increase of n-butanol extract content from Sanguisorba officinalis L., the growth inhibition ratio increased gradually, which were all significantly higher than that of the blank control group (P<0.05). The cell apoptotic rate and intracellular ROS content were significantly higher in the HepG2 cells treated by different concentrations (200, 400, 600μg·mL-1) of n-butanol extract from Sanguisorba officinalis L. for 48h than those of the blank control group. In addition, with the increase of the concentrations of n-butanol extract from Sanguisorba of-ficinalis L., the cell apoptotic rate and intracellular ROS content increased gradually. Conclusion The n-butanol extract from Sanguisorba officinalis L. could suppress the proliferation and promote the apoptosis of HepG2 cells in a concentration-depend-ent manner, and this may be related to its promotion in intracellular ROS production.