中华口腔医学研究杂志（电子版）
中華口腔醫學研究雜誌（電子版）
중화구강의학연구잡지（전자판）
CHINESE JOURNAL OF STOMATOLOGICAL RESEARCH(ELECTRONIC VERSION)
2014年
2期
118-122
,共5页
张宁宁%赵婷婷%何倩婷%丁学强%刘中华%王安训
張寧寧%趙婷婷%何倩婷%丁學彊%劉中華%王安訓
장저저%조정정%하천정%정학강%류중화%왕안훈
舌鳞癌%MTUS1/ATIP1%增殖%凋亡
舌鱗癌%MTUS1/ATIP1%增殖%凋亡
설린암%MTUS1/ATIP1%증식%조망
Tongue squmous cell carcinoma%MTUS1/ATIP1%Proliferation%Apoptosis
目的：探讨过表达MTUS1/ATIP1对舌鳞癌细胞增殖及凋亡的影响。方法检测人舌鳞癌系UM1、SCC鄄9、SCC鄄15、Tca8113细胞株中MTUS1的表达水平。应用含ATIP1片段的质粒转染舌鳞癌细胞，48 h后MTT检测舌鳞癌细胞的增殖能力；应用流式细胞仪技术和细胞免疫荧光技术检测细胞周期和细胞凋亡率；Western blot检测舌鳞癌细胞中MTUS1、p53、ERK1/2的表达情况。结果转染MTUS1/ATIP1后细胞的增殖明显受到抑制，其抑制率约为40％（t=0.023，P＜0.05）；高表达MTUS1/ATIP1可导致舌鳞癌细胞株G1期阻滞（G1期：t=0.032，G2期：t=0.036，S期：t=0.027，P＜0.05）并诱导细胞凋亡率的明显升高，差异具有统计学意义（t=0.005，P＜0.05）。 Western blot检测显示，转染MTUS1/ATIP1后ERK的表达升高，磷酸化的ERK表达下降，p53的表达升高。结论 MTUS1可抑制舌鳞癌细胞的增殖，诱导细胞凋亡。
目的：探討過錶達MTUS1/ATIP1對舌鱗癌細胞增殖及凋亡的影響。方法檢測人舌鱗癌繫UM1、SCC鄄9、SCC鄄15、Tca8113細胞株中MTUS1的錶達水平。應用含ATIP1片段的質粒轉染舌鱗癌細胞，48 h後MTT檢測舌鱗癌細胞的增殖能力；應用流式細胞儀技術和細胞免疫熒光技術檢測細胞週期和細胞凋亡率；Western blot檢測舌鱗癌細胞中MTUS1、p53、ERK1/2的錶達情況。結果轉染MTUS1/ATIP1後細胞的增殖明顯受到抑製，其抑製率約為40％（t=0.023，P＜0.05）；高錶達MTUS1/ATIP1可導緻舌鱗癌細胞株G1期阻滯（G1期：t=0.032，G2期：t=0.036，S期：t=0.027，P＜0.05）併誘導細胞凋亡率的明顯升高，差異具有統計學意義（t=0.005，P＜0.05）。 Western blot檢測顯示，轉染MTUS1/ATIP1後ERK的錶達升高，燐痠化的ERK錶達下降，p53的錶達升高。結論 MTUS1可抑製舌鱗癌細胞的增殖，誘導細胞凋亡。
목적：탐토과표체MTUS1/ATIP1대설린암세포증식급조망적영향。방법검측인설린암계UM1、SCC견9、SCC견15、Tca8113세포주중MTUS1적표체수평。응용함ATIP1편단적질립전염설린암세포，48 h후MTT검측설린암세포적증식능력；응용류식세포의기술화세포면역형광기술검측세포주기화세포조망솔；Western blot검측설린암세포중MTUS1、p53、ERK1/2적표체정황。결과전염MTUS1/ATIP1후세포적증식명현수도억제，기억제솔약위40％（t=0.023，P＜0.05）；고표체MTUS1/ATIP1가도치설린암세포주G1기조체（G1기：t=0.032，G2기：t=0.036，S기：t=0.027，P＜0.05）병유도세포조망솔적명현승고，차이구유통계학의의（t=0.005，P＜0.05）。 Western blot검측현시，전염MTUS1/ATIP1후ERK적표체승고，린산화적ERK표체하강，p53적표체승고。결론 MTUS1가억제설린암세포적증식，유도세포조망。
Objective To investigate the role of MTUS1/ATIP1 in suppression proliferation and induce apoptosis of oral tongue squmous cell carcinoma . Methods Investigate the expression levels of MTUS1 in human tongue cell carcinoma cell lines (UM1, SCC-15, SCC-9, Tca8113). Tongue squamous cancer cell were transfected with a ATIP1 expression vector for 48 h . The Proliferation analysis was performed with MTT assay. Flow cytometry and immunofluorescence technique were employed to measure cell cycle and apoptosis of tongue cell carcinoma cells. The expression levels of proteins, MTUS1, p53, ERK1/2, in tongue cell carcinoma cells transfected with ATIP1 were detected by Western blotting . Results In vitro functional study showed that over-expression of MTUS1/ATIP1 significantly increased the apoptosis rate (P<0.05), led to G1 arrest and decreased the proliferation activity, with an inhibition rate of about 40% (P < 0.05) in OTSCC cell lines. The over-expression of MTUS1/ATIP1 induced the reduction of phosphor-ylation of ERK and up-regulation of p53 and ERK . Conclusion MTUS1/ATIP1 is suppose to be associated with inhibit proliferation and induce apoptosis in oral tongue squmous cell carcinoma.
