CAJ | 학술논문

目的：构建牙本质混合层原位再矿化诱导模型，并从微观形态学角度探讨其矿化效果，为仿生再矿化技术的临床应用提供实验依据。方法采用5 mm ×5 mmⅠ型胶原海绵块作为3D胶原支架，结合电镜观察和傅里叶红外光谱分析技术，快速评估成核诱导物多聚磷酸钠（STTP）和硅酸盐水门汀树脂的仿生矿化诱导潜能。在此基础上，将仿生矿化技术与牙本质粘接程序结合，以STTP作为治疗性底剂应用于酸蚀脱矿牙本质面，在完成常规粘接处理后以硅酸盐水门汀树脂为洞衬剂，构建临床相关的混合层原位再矿化模型。采用透射电子显微镜观察矿化1～3个月后树脂牙本质粘接界面再矿化区域的分布和再矿化程度。结果矿化诱导28 d后的3D胶原样本，纤维内可见磷灰石纳米晶体的有序沉积，纤维重现了与天然矿化胶原结构类似的横纹特征。红外光谱分析结果显示，在900～1200 cm-1和500～600 cm-1区域，矿化胶原基质出现与纯羟基磷灰石特征峰相吻合的吸收峰，提示矿化胶原中形成的无机物是磷灰石。混合层原位矿化诱导1～3个月后，混合层内可见纤维内再矿化现象的存在，纳米晶体在胶原纤维内呈现迭序排列特征。结论在牙体粘接修复过程中，以矿化诱导物STTP和硅酸盐水门汀树脂作为治疗性底剂和洞衬剂，能使混合层内树脂渗透不良的胶原基质发生纤维内矿化。通过混合层原位再矿化模型的成功构建，初步证实仿生矿化技术应用于临床树脂牙本质粘接界面损伤修复的可行性，建立了研究方法学，为仿生再矿化技术的最终临床应用提供充分的科学依据。
목적：구건아본질혼합층원위재광화유도모형，병종미관형태학각도탐토기광화효과，위방생재광화기술적림상응용제공실험의거。방법채용5 mm ×5 mmⅠ형효원해면괴작위3D효원지가，결합전경관찰화부리협홍외광보분석기술，쾌속평고성핵유도물다취린산납（STTP）화규산염수문정수지적방생광화유도잠능。재차기출상，장방생광화기술여아본질점접정서결합，이STTP작위치료성저제응용우산식탈광아본질면，재완성상규점접처리후이규산염수문정수지위동츤제，구건림상상관적혼합층원위재광화모형。채용투사전자현미경관찰광화1～3개월후수지아본질점접계면재광화구역적분포화재광화정도。결과광화유도28 d후적3D효원양본，섬유내가견린회석납미정체적유서침적，섬유중현료여천연광화효원결구유사적횡문특정。홍외광보분석결과현시，재900～1200 cm-1화500～600 cm-1구역，광화효원기질출현여순간기린회석특정봉상문합적흡수봉，제시광화효원중형성적무궤물시린회석。혼합층원위광화유도1～3개월후，혼합층내가견섬유내재광화현상적존재，납미정체재효원섬유내정현질서배렬특정。결론재아체점접수복과정중，이광화유도물STTP화규산염수문정수지작위치료성저제화동츤제，능사혼합층내수지삼투불량적효원기질발생섬유내광화。통과혼합층원위재광화모형적성공구건，초보증실방생광화기술응용우림상수지아본질점접계면손상수복적가행성，건립료연구방법학，위방생재광화기술적최종림상응용제공충분적과학의거。
Objective To test the hypothesis that the application of a sodium tripolyphosphate (STTP) primer and a Portland cement-based lining composite on hybrid layers can induce intrafibrillar remineralization in the presence of the amorphous calcium phosphate (ACP)-stabilization analog supplied in simulated body fluid (SBF). Methods Together with Portland cement-based composite, biomimetic remineralization system was built by phosphorylating collagen with 0 .245 mol/L STTP for 5 min and by adding polyacrylic acid ( PAA ) to SBF . Based on these results , a in-situ mineralization scheme was developed for chemical phosphorylation of acid-etched dentin with 0 .245 mol/L STTP, followed by infiltration of the STTP-treated collagen matrix with One-Step . Resin dentin interfaces were then capped with a Portland cement-based hydrophilic composite a liner , and remineralized in SBF with the use of PAA as an ACP-stabilization analog. Remineralized resin-dentin interfaces were examined unstained using transmission electron microscopy (TEM). Results Periodic nanocomposite assembly with collagen sponge as a template was demonstrated with TEM using a Portland cement-based resin composite and a 5-minute STTP phosphorylation. Apatite was detected within the collagen at 72 h, and heavily deposited in collagen fibrils with periodically arranged intrafibrillar apatite platelets at 28 d . Infrared spectrum of the mineralized collagen sponge demonstrated characteristic hydroxyl apatite peaks at 900~1200 cm -1 and 500~600 cm-1. The results of that model were further validated by complete remineralization of the hybrid layers treated with 0.245 mol/L STTP as a therapeutic primer and lined with a Portland cement-based lining composite, and with the ACP-stabilization analog supplied in SBF. Conclusions The STTP primer and the Portland cement-based lining composite employed in this study may be used with the ACP-stabilization analogy for in-situ remineralization of hybrid layer . This provides a potential delivery system for realization of the goal of biomimetic remineralization of dentin hybrid layer .