CAJ | 학술논문

目的：探讨不同浓度的富血小板血浆（PRP）支架在体内诱导牙髓组织再生的能力。方法将小型猪乳牙牙根段进行化学预备，采用二次离心法制备PRP，根据注入根管中的成分不同将研究分为4组：（1）阴性对照组，即全血组；（2）100％ PRP组；（3）50％ PRP组；（4）空白组，即空的牙根段；每组5个样本，分别植入裸鼠背部皮下，于术后5周处死动物，取出样本进行组织学观察。结果植入5周后，100％ PRP组根管内充满了炎性细胞，50％ PRP组根管内有少量牙髓样的组织生成。结论合适浓度的PRP作为生物支架在体内再生牙髓样的组织是可行的。
목적：탐토불동농도적부혈소판혈장（PRP）지가재체내유도아수조직재생적능력。방법장소형저유아아근단진행화학예비，채용이차리심법제비PRP，근거주입근관중적성분불동장연구분위4조：（1）음성대조조，즉전혈조；（2）100％ PRP조；（3）50％ PRP조；（4）공백조，즉공적아근단；매조5개양본，분별식입라서배부피하，우술후5주처사동물，취출양본진행조직학관찰。결과식입5주후，100％ PRP조근관내충만료염성세포，50％ PRP조근관내유소량아수양적조직생성。결론합괄농도적PRP작위생물지가재체내재생아수양적조직시가행적。
Objective To evaluate the in vivo dental pulp regeneration capacity of various concentration of the platelet-rich plasma (PRP). Methods The tooth roots of minipigs were prepared by chemical methods. PRP was extracted by two-step centrifugation. The roots were divided into four groups according to the kinds of scaffold injected into the root canals (5 roots each group): (1)control group, whole blood; (2)100% PRP; (3)50% PRP; (4)blank group: root fragments with empty canal space. The roots were implanted subcutaneously in nude mice. The animals were sacrificed after 5 weeks. The samples were taken for histological examination . Results The newly formed pulp-like tissues were observed in the roots which filled 50% PRP scaffolds in canals . The group of 100% PRP scaffolds were full of inflammatory cells. Conclusions It is feasible for the platelet-rich plasma with proper concentration as scaffolds to induce dental pulp regeneration in vivo.