内科
內科
내과
INTERNAL MEDICINE OF CHINA
2014年
2期
124-127
,共4页
急性早幼粒细胞白血病%HL-60 细胞%柔红霉素%IRF1%IRF8%IRF9%细胞凋亡
急性早幼粒細胞白血病%HL-60 細胞%柔紅黴素%IRF1%IRF8%IRF9%細胞凋亡
급성조유립세포백혈병%HL-60 세포%유홍매소%IRF1%IRF8%IRF9%세포조망
Acute promyelocytic leukemia%HL-60 cell line%Daunorubicin%Interferon regulatory factor 1,Interferon regulatory factor 8,Interferon regulatory factor 9%Cell apoptosis
目的：研究柔红霉素(Daunorubicin,DNR)对人急性早幼粒细胞白血病(APL)细胞株 HL-60细胞中干扰素调节因子1(IRF1)-mRNA、干扰素调节因子8(IRF8)-mRNA、干扰素调节因子9(IRF9)-mRNA表达的影响。方法将 HL-60细胞株设为 HL-60组、HL-60+DNR组,同时取3例正常人外周血白细胞为 NC 组。HL-60组为未加药处理组, HL-60+DNR组为小剂量DNR持续作用 HL-60细胞10 d。采用实时荧光定量聚合酶链反应(RT-PCR)法检测 IRF1-mRNA、IRF8-mRNA、IRF9-mRNA 转录水平,每组实验重复3次。结果与 NC组相比,IRF1-mRNA、IRF8-mR-NA转录水平在 HL-60组显著下调(P<0.05),而 HL-60+DNR组显著上调(P<0.05)；与 NC组相比,IRF9-mRNA转录水平在 HL-60+DNR显著上调(P<0.05),在 HL-60组则表现为下调,但无统计学差异(P>0.05)。结论 DNR可上调 HL-60细胞中的IRF1、IRF8、IRF9表达水平。APL经DNR治疗后,可能通过上调IRF1、IRF8、IRF9的表达诱导白血病细胞的凋亡,促进其成熟,促进病情的缓解。
目的：研究柔紅黴素(Daunorubicin,DNR)對人急性早幼粒細胞白血病(APL)細胞株 HL-60細胞中榦擾素調節因子1(IRF1)-mRNA、榦擾素調節因子8(IRF8)-mRNA、榦擾素調節因子9(IRF9)-mRNA錶達的影響。方法將 HL-60細胞株設為 HL-60組、HL-60+DNR組,同時取3例正常人外週血白細胞為 NC 組。HL-60組為未加藥處理組, HL-60+DNR組為小劑量DNR持續作用 HL-60細胞10 d。採用實時熒光定量聚閤酶鏈反應(RT-PCR)法檢測 IRF1-mRNA、IRF8-mRNA、IRF9-mRNA 轉錄水平,每組實驗重複3次。結果與 NC組相比,IRF1-mRNA、IRF8-mR-NA轉錄水平在 HL-60組顯著下調(P<0.05),而 HL-60+DNR組顯著上調(P<0.05)；與 NC組相比,IRF9-mRNA轉錄水平在 HL-60+DNR顯著上調(P<0.05),在 HL-60組則錶現為下調,但無統計學差異(P>0.05)。結論 DNR可上調 HL-60細胞中的IRF1、IRF8、IRF9錶達水平。APL經DNR治療後,可能通過上調IRF1、IRF8、IRF9的錶達誘導白血病細胞的凋亡,促進其成熟,促進病情的緩解。
목적：연구유홍매소(Daunorubicin,DNR)대인급성조유립세포백혈병(APL)세포주 HL-60세포중간우소조절인자1(IRF1)-mRNA、간우소조절인자8(IRF8)-mRNA、간우소조절인자9(IRF9)-mRNA표체적영향。방법장 HL-60세포주설위 HL-60조、HL-60+DNR조,동시취3례정상인외주혈백세포위 NC 조。HL-60조위미가약처리조, HL-60+DNR조위소제량DNR지속작용 HL-60세포10 d。채용실시형광정량취합매련반응(RT-PCR)법검측 IRF1-mRNA、IRF8-mRNA、IRF9-mRNA 전록수평,매조실험중복3차。결과여 NC조상비,IRF1-mRNA、IRF8-mR-NA전록수평재 HL-60조현저하조(P<0.05),이 HL-60+DNR조현저상조(P<0.05)；여 NC조상비,IRF9-mRNA전록수평재 HL-60+DNR현저상조(P<0.05),재 HL-60조칙표현위하조,단무통계학차이(P>0.05)。결론 DNR가상조 HL-60세포중적IRF1、IRF8、IRF9표체수평。APL경DNR치료후,가능통과상조IRF1、IRF8、IRF9적표체유도백혈병세포적조망,촉진기성숙,촉진병정적완해。
Objective To explore the effect of daunorubicin(DNR)on the expression of IRF1-mRNA,IRF8-mRNA and IRF9-mRNA in acute promyelocytic leukemia cell line of HL-60 cell.Methods HL-60 cell line was set to HL-60 group,HL-60+DNR group,and the peripheral white blood cells separated from the blood of normal person served as NC group.The HL-60+DNR group was exposed to small doses of DNR every two day for 10 days,while the HL-60 group received no treatment.IRF1-mRNA,IRF8-mRNA and IRF9-mRNA were detected by real-time reverse transcription polymerase chain reaction(RT-PCR).The analysis for each group was performed for three times.Results The expression of IRF1-mRNA and IRF8-mRNA in the HL-60 group were markedly down-regulated compared to the NC group(P<0.05),but were markedly up-regulated in the HL-60+DNR group(P<0.05);compared to the NC group,the expres-sion of IRF9-mRNA in the HL-60+DNR group was markedly up-regulated (P<0.05),and down-regulated in the HL-60 group,but there was no significant difference between the two groups (P>0.05).Conclusions DNR can up-reg-ulate the expression of IRF1、IRF8、IRF9 in HL-60 cell line.DNR may induce apoptosis and promote maturation on leuke-mia cells and promote remission in APL patients.
