CAJ | 학술논문

目的：研究丙酮酸盐对阿尔茨海默病(Alzheimer’s disease, AD)模型大鼠学习记忆的影响并探讨其与海马神经细胞死亡的关系。方法将36只wistar大鼠随机分为空白对照组、AD模型组和丙酮酸盐治疗组，根据实验要求给药处理6周，同时观察各组大鼠一般情况，Morris水迷宫实验检测大鼠学习记忆能力，苏木精-伊红（hematoxylin and eosin, HE）染色检测海马神经细胞死亡，利用2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA)来检测海马CA1区内活性氧（reactive oxygen species, ROS）聚集情况。结果丙酮酸盐治疗组与AD模型组比较，一般情况均明显改善，逃避潜伏期均明显缩短(41.37±6.24,31.67±7.26,24.26±7.16,20.45±5.72,19.36±5.89,均P<0.05)；原平台象限活动时间明显增加(50.36±7.46 s, P<0.05)，穿越原平台次数明显增多(3.98±1.23次/2 min，P<0.05)，海马CA1区锥体神经细胞死亡显著减少(P<0.05)，海马神经细胞内ROS的水平也显著降低（P<0.05）。结论丙酮酸盐可能通过降低海马组织内ROS的产生，减少海马神经细胞死亡，从而提高AD模型大鼠的学习记忆水平。
목적：연구병동산염대아이자해묵병(Alzheimer’s disease, AD)모형대서학습기억적영향병탐토기여해마신경세포사망적관계。방법장36지wistar대서수궤분위공백대조조、AD모형조화병동산염치료조，근거실험요구급약처리6주，동시관찰각조대서일반정황，Morris수미궁실험검측대서학습기억능력，소목정-이홍（hematoxylin and eosin, HE）염색검측해마신경세포사망，이용2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA)래검측해마CA1구내활성양（reactive oxygen species, ROS）취집정황。결과병동산염치료조여AD모형조비교，일반정황균명현개선，도피잠복기균명현축단(41.37±6.24,31.67±7.26,24.26±7.16,20.45±5.72,19.36±5.89,균P<0.05)；원평태상한활동시간명현증가(50.36±7.46 s, P<0.05)，천월원평태차수명현증다(3.98±1.23차/2 min，P<0.05)，해마CA1구추체신경세포사망현저감소(P<0.05)，해마신경세포내ROS적수평야현저강저（P<0.05）。결론병동산염가능통과강저해마조직내ROS적산생，감소해마신경세포사망，종이제고AD모형대서적학습기억수평。
Objective To study the effects of pyruvate on learning, memory and neuronal cell death of the hippo-campus in a rat model of Alzheimer’s disease (AD). Methods A total of 36 rats were randomly divided into control group, AD model group,and pyruvate group. General condition was observed after 6-week dipsacus treatment. Morris water maze was used to determine 1earning and memory in animals.Hematoxylin and eosin (H-E) staining was used to quantify neuronal death in the hippocampus. The reactive oxygen species (ROS) was assessed by using 2′, 7′-dichlorodi-hydrofluorescein diacetate (H2DCF-DA). Results Compared with AD model group, pyruvate group showed improved gen-eral condition, shortened escape latency (41.37±6.24, 31.67±7.26, 24.26±7.16, 20.45±5.72, 19.36±5.89,respectively P<0.05),increased activity time on the former platform quadrant (50.36 ± 7.46 s, P<0.05),increased number of crossing the former platform (3.98±1.23 time/2 min,P<0.05),reduced ROS levels and decreased neuronal cell death in CA1 of the hippocampus decreased (respectively, P<0.05). Conclusions Pyruvate can improve the learning and memory and de-crease the neuronal cell death through preventing the ROS production in AD rats.