CAJ | 학술논문

目的观察磺脲类药物对兔心肌缺血再灌注损伤后适应影响及其机制。方法将30只新西兰大白兔随机分为5组：（1）假手术组，仅穿线不作冠状动脉结扎；（2）缺血-再灌注组，缺血45分钟，再灌注2小时；（3）后适应组，缺血45分钟，然后行短暂灌注30秒，缺血30秒，共3次，再行全面的2小时再灌注；（4）格列美脲组，实验开始时外周静脉注射格列美脲0.2mg/kg ，其余操作同后适应组；（5）格列本脲组，实验开始时外周注射格列本脲0.5mg/kg ，其余操作同后适应组。实验结束时测各组血清肌钙蛋白含量及心肌梗死面积。结果肌钙蛋白含量假手术组明显低于各手术组，后适应组与格列美脲组显著低于缺血再灌注组与格列本脲组，差异均有统计学意义（ P＜0.01），而后适应组与格列美脲组及缺血再灌注组与格列本脲组差异均无统计学意义（ P＞0.05）。心肌梗死面积后适应组、格列美脲组明显低于缺血再灌注组及格列本脲组，差异有统计学意义（ P＜0.01）；后适应组与格列美脲组、缺血再灌注组与格列本脲组之间差异无统计学意义（ P＞0.05）。结论格列本脲可消除后适应兔心肌缺血再灌注损伤的保护作用，而格列美脲却对后适应保护作用无影响。
목적관찰광뇨류약물대토심기결혈재관주손상후괄응영향급기궤제。방법장30지신서란대백토수궤분위5조：（1）가수술조，부천선불작관상동맥결찰；（2）결혈-재관주조，결혈45분종，재관주2소시；（3）후괄응조，결혈45분종，연후행단잠관주30초，결혈30초，공3차，재행전면적2소시재관주；（4）격렬미뇨조，실험개시시외주정맥주사격렬미뇨0.2mg/kg ，기여조작동후괄응조；（5）격렬본뇨조，실험개시시외주주사격렬본뇨0.5mg/kg ，기여조작동후괄응조。실험결속시측각조혈청기개단백함량급심기경사면적。결과기개단백함량가수술조명현저우각수술조，후괄응조여격렬미뇨조현저저우결혈재관주조여격렬본뇨조，차이균유통계학의의（ P＜0.01），이후괄응조여격렬미뇨조급결혈재관주조여격렬본뇨조차이균무통계학의의（ P＞0.05）。심기경사면적후괄응조、격렬미뇨조명현저우결혈재관주조급격렬본뇨조，차이유통계학의의（ P＜0.01）；후괄응조여격렬미뇨조、결혈재관주조여격렬본뇨조지간차이무통계학의의（ P＞0.05）。결론격렬본뇨가소제후괄응토심기결혈재관주손상적보호작용，이격렬미뇨각대후괄응보호작용무영향。
Objective To explore the effects of sulfonylurea on myocardial ischemia_reperfusion injury in rabbits.Methods 30 healthy New Zealand rabbits were randomly divided into 5 groups:(1 )sham operation group (coronary artery ligation ,CAL );(2 ) ischemia reperfusion injury group (IRI) ,ischemia for 45 minutes and reperfusion for 2 hours;(3) postconditioning (Post C) group ,after is-chemia for 45 minutes ,30 seconds reperfusion ,30 seconds occlusion of coronary artery and repeated for 3 times ,then reperfusion for 2 hours;(4) glimepiride group ,IV 0.2mg/kg of glimepiride through periphery vein ,then the same process conducted as Post C group;(5 )glibenclamide group ,IV 0.5mg/kg of glibenclamide through periphery vein ,then the same process conducted as Post C group.The infarct size and the content of cTnI were measured at the end of experiment.Results The content of cTnI in sham opera-tion group was significantly lower compared with the other groups.The content of cTnI in Post C and Glimepiride group were signifi-cantly lower than IRI and glibenclamide groups ( P< 0.01 ). There was no significant difference between Post C and glimepiride group ,IRI and glibenclamide group.The infarct size of Post C and glimepiride group were significantly smaller compared with the IRI and glibenclamide group ;there was no difference between Post C group and glimepiride group ,and there was also no difference be-tween IRI group and glibenclamide group.Conclusions Postconditioning may provide myocardial protection in rabbits in IRI.This ef-fect can be abolished by glibenclamide but not by glimepiridei .