CAJ | 학술논문

目的：探讨滴鼻和雾化两种不同激发方式对小鼠支气管哮喘模型气道炎症的影响，为研究和优化支气管哮喘动物模型提供实验和理论依据。方法选择鸡卵清蛋白（OVA）致敏的BALB/c小鼠，分别采用滴鼻和雾化两种不同方式进行激发，复制哮喘模型，观察支气管肺泡灌洗液（BALF）中白细胞总数和细胞分类计数，ELISA方法检测BALF上清中IL-4、IL-13含量，常规病理切片苏木精-伊红（HE）染色观察肺内炎症情况。结果滴鼻组小鼠BALF中白细胞总数最高，其次为雾化组小鼠；且滴鼻组小鼠嗜酸性粒细胞总数及中性粒细胞总数亦明显高于雾化组与正常组，差异有统计学意义（P＜0.05）。滴鼻组小鼠BALF中 IL-4、IL-13水平明显高于雾化组与正常组小鼠，差异有统计学意义（P＜0.05）；雾化组小鼠BALF中IL-4、IL-13水平高于正常组小鼠，差异有统计学意义（P＜0.05）。滴鼻组肺组织炎症反应最明显，并可见上皮细胞破坏，杯状细胞增生，支气管和血管周围有大量炎症细胞浸润，以嗜酸性粒细胞和淋巴细胞为主。雾化组炎症程度较轻，正常对照组无明显炎症改变。结论雾化吸入和经鼻滴入的激发方式都可以成功制作出支气管哮喘模型，经鼻滴入方式可以引起肺部更加明显的炎症浸润及细胞因子表达，为哮喘研究提供更好的选择。
목적：탐토적비화무화량충불동격발방식대소서지기관효천모형기도염증적영향，위연구화우화지기관효천동물모형제공실험화이론의거。방법선택계란청단백（OVA）치민적BALB/c소서，분별채용적비화무화량충불동방식진행격발，복제효천모형，관찰지기관폐포관세액（BALF）중백세포총수화세포분류계수，ELISA방법검측BALF상청중IL-4、IL-13함량，상규병리절편소목정-이홍（HE）염색관찰폐내염증정황。결과적비조소서BALF중백세포총수최고，기차위무화조소서；차적비조소서기산성립세포총수급중성립세포총수역명현고우무화조여정상조，차이유통계학의의（P＜0.05）。적비조소서BALF중 IL-4、IL-13수평명현고우무화조여정상조소서，차이유통계학의의（P＜0.05）；무화조소서BALF중IL-4、IL-13수평고우정상조소서，차이유통계학의의（P＜0.05）。적비조폐조직염증반응최명현，병가견상피세포파배，배상세포증생，지기관화혈관주위유대량염증세포침윤，이기산성립세포화림파세포위주。무화조염증정도교경，정상대조조무명현염증개변。결론무화흡입화경비적입적격발방식도가이성공제작출지기관효천모형，경비적입방식가이인기폐부경가명현적염증침윤급세포인자표체，위효천연구제공경호적선택。
Objective To investigate the effect of two different methods of challenge ,intranasal or aerosol exposure ,on air-way inflammation in murine asthma models ,and provide experimental and theoretical basis for the study and optimization of bronchial asthma in animal models.Methods Ovalbumin(OVA)-sensitive BALB/c mice were challenged to set up murine mod-els of atopic asthma by intranasal or aerosol exposure to OVA.The number of leukocytes and cell classifications in bronchoalve-olar lavage fluid (BALF) were measured.The concentrations of IL-4 and IL-13 in the supernatant of BALF were determined by ELISA and the histological changes in the lung by hematoxylin and eosin (HE) staining.Results The total leukocytes were highest in the intranasal group.The number of eosinophils and neutrophils in the intranasal group were higher than that in the aerosol group and normal group (P< 0.05).The levels of IL-4 and IL-13 were significantly increased in the supernatant of BALF in the intranasal group when compared with the aerosol group and normal group (P<0.05).The levels of IL-4 and IL-13 in the aerosol group were higher than those in the normal group (P<0.05).The lung tissue inflammation presented destruction of epithelial cells ,hyperplasia of goblet cells and infiltration of large numbers of inflammatory cells (predominantly eosinophils and lymphocytes ).It was most obvious in the intranasal group and no obvious inflammation was observed in the normal group.Conclusion Asthmatic models can be established successfully by both of intranasal and aerosol challenge and the in-tranasal administration triggers more serious airway inflammation and higher cytokines expression than aerosol challenge ,provi-ding better choice for the study of asthma.