中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
3期
228-234
,共7页
赵志强%乔瑞洁%王欣茹%王浩%杨进%权怡%史晓玲%刘佳%林纪胜%谢贵林
趙誌彊%喬瑞潔%王訢茹%王浩%楊進%權怡%史曉玲%劉佳%林紀勝%謝貴林
조지강%교서길%왕흔여%왕호%양진%권이%사효령%류가%림기성%사귀림
A群C群脑膜炎球菌多糖疫苗%免疫原性%酶联免疫吸附试验%血清杀菌试验( SBA )
A群C群腦膜炎毬菌多糖疫苗%免疫原性%酶聯免疫吸附試驗%血清殺菌試驗( SBA )
A군C군뇌막염구균다당역묘%면역원성%매련면역흡부시험%혈청살균시험( SBA )
Meningococcal serogroups A and C polysaccharide vaccine%Immuogenicity%Enzyme-linked immunosorbent assay (ELISA)%Serum bactericidal assay ( SBA)
目的：对A群C群脑膜炎球菌多糖疫苗在5～59岁健康人群中的免疫原性进行再评价。方法对2005年在广西河池市进行的临床试验中采集的血清，采用国际标准化的酶联免疫吸附试验（ELISA）和血清体外杀菌试验（SBA）方法检测血清中抗A群C群脑膜炎球菌荚膜多糖的特异性IgG抗体含量和对A群C群脑膜炎球菌的抗体杀菌功能。结果免疫前、后，A群血清中抗荚膜多糖特异性IgG抗体平均几何浓度（GMC）分别为23．66μg/ml和78．83μg/ml （P＜0．05），C群分别为1．23μg/ml和51．25μg/ml （P＜0．05）；免疫前、后血清中抗荚膜多糖IgG抗体浓度≥2μg/ml的比例，A群分别为99％和100％（P＞0．05），C群为20％和99％（P＜0．05）；免疫后较免疫前抗荚膜多糖IgG抗体浓度≥4倍升高的比例，A群为34％和C群为100％。免疫前、后血清中针对脑膜炎球菌的杀菌抗体平均几何滴度（GMT），A群分别为1164和5530（P＜0．05），C群分别为4和6225（P＜0．05）；免疫前、后血清杀菌抗体GMT≥128的比例，A群分别为91％和99％（P＞0．05），C群分别为14％和96％（ P＜0．05）；免疫后较免疫前血清杀菌抗体滴度≥4倍升高的比例分别为A群53％和C群100％。免疫前、后血清中抗荚膜多糖IgG抗体浓度和杀菌抗体滴度之间的Pearson相关系数，A群分别为r＝0．15和r＝0．23，C群r＝-0．14和r＝0．58（P＜0．05）。结论本研究以标准化检测方法产生的具体免疫原性数据，证明A群C群脑膜炎球菌多糖疫苗良好的免疫原性。
目的：對A群C群腦膜炎毬菌多糖疫苗在5～59歲健康人群中的免疫原性進行再評價。方法對2005年在廣西河池市進行的臨床試驗中採集的血清，採用國際標準化的酶聯免疫吸附試驗（ELISA）和血清體外殺菌試驗（SBA）方法檢測血清中抗A群C群腦膜炎毬菌莢膜多糖的特異性IgG抗體含量和對A群C群腦膜炎毬菌的抗體殺菌功能。結果免疫前、後，A群血清中抗莢膜多糖特異性IgG抗體平均幾何濃度（GMC）分彆為23．66μg/ml和78．83μg/ml （P＜0．05），C群分彆為1．23μg/ml和51．25μg/ml （P＜0．05）；免疫前、後血清中抗莢膜多糖IgG抗體濃度≥2μg/ml的比例，A群分彆為99％和100％（P＞0．05），C群為20％和99％（P＜0．05）；免疫後較免疫前抗莢膜多糖IgG抗體濃度≥4倍升高的比例，A群為34％和C群為100％。免疫前、後血清中針對腦膜炎毬菌的殺菌抗體平均幾何滴度（GMT），A群分彆為1164和5530（P＜0．05），C群分彆為4和6225（P＜0．05）；免疫前、後血清殺菌抗體GMT≥128的比例，A群分彆為91％和99％（P＞0．05），C群分彆為14％和96％（ P＜0．05）；免疫後較免疫前血清殺菌抗體滴度≥4倍升高的比例分彆為A群53％和C群100％。免疫前、後血清中抗莢膜多糖IgG抗體濃度和殺菌抗體滴度之間的Pearson相關繫數，A群分彆為r＝0．15和r＝0．23，C群r＝-0．14和r＝0．58（P＜0．05）。結論本研究以標準化檢測方法產生的具體免疫原性數據，證明A群C群腦膜炎毬菌多糖疫苗良好的免疫原性。
목적：대A군C군뇌막염구균다당역묘재5～59세건강인군중적면역원성진행재평개。방법대2005년재엄서하지시진행적림상시험중채집적혈청，채용국제표준화적매련면역흡부시험（ELISA）화혈청체외살균시험（SBA）방법검측혈청중항A군C군뇌막염구균협막다당적특이성IgG항체함량화대A군C군뇌막염구균적항체살균공능。결과면역전、후，A군혈청중항협막다당특이성IgG항체평균궤하농도（GMC）분별위23．66μg/ml화78．83μg/ml （P＜0．05），C군분별위1．23μg/ml화51．25μg/ml （P＜0．05）；면역전、후혈청중항협막다당IgG항체농도≥2μg/ml적비례，A군분별위99％화100％（P＞0．05），C군위20％화99％（P＜0．05）；면역후교면역전항협막다당IgG항체농도≥4배승고적비례，A군위34％화C군위100％。면역전、후혈청중침대뇌막염구균적살균항체평균궤하적도（GMT），A군분별위1164화5530（P＜0．05），C군분별위4화6225（P＜0．05）；면역전、후혈청살균항체GMT≥128적비례，A군분별위91％화99％（P＞0．05），C군분별위14％화96％（ P＜0．05）；면역후교면역전혈청살균항체적도≥4배승고적비례분별위A군53％화C군100％。면역전、후혈청중항협막다당IgG항체농도화살균항체적도지간적Pearson상관계수，A군분별위r＝0．15화r＝0．23，C군r＝-0．14화r＝0．58（P＜0．05）。결론본연구이표준화검측방법산생적구체면역원성수거，증명A군C군뇌막염구균다당역묘량호적면역원성。
Objective To re-evaluate the immunogenicity of meningococcal serogroups A and C polysaccharide vaccine among a healthy population of age 5 to 59.Methods Pre and post-vaccination ser-um samples were collected from the subjects involved in a randomized , controlled clinical trial conducted in 2005 at Hechi, Guangxi, China.Enzyme-linked immunosorbent assay (ELISA) was performed for the quan-titative detection of specific anti-capsule IgG antibody against meningococcal serogroups A and C in serum samples.Serum bactericidal assay ( SBA) was used to measure the bactericidal antibody activity against se-rougroups A and C bacterial strains .Results Geometric mean concentrations (GMCs) of specific anti-cap-sule IgG antibody were 23.66 μg/ml and 78.83 μg/ml for serogroup A (P<0.05), and 1.23 μg/ml and 51.25 μg/ml for serogroup C (P<0.05) in serum samples of pre and post-vaccination, respectively.Be-fore immunization , 99% of serum samples ( 97/98 ) showed serogroup A-specific IgG concentration ≥2μg/ml, which reached up to 100%(98/98) after vaccination (P>0.05).The percentage of serum sam-ples with serogroup C-specific IgG concentration ≥2 μg/ml rose from 20%to 99% after vaccination ( P<0.05).The ratio of positive serum samples with at least four times of increases in concentration of specific IgG against serogroup A and serogroup C after vaccine immunization were 34% and 100%, respectively . The rSBA geometric mean titers ( GMTs) for serogroups A and C in serum sample of post-vaccination were respectively elevated from 1164 to 5530 (P<0.05), and from 4 to 6225 (P<0.05).The percentage of se-rum samples with rSBA GMTs≥128 increased from 91% to 99% for serogroup A (P>0.05), and from 14%to 96%for serogroup C (P<0.05) after vaccination.The rSBA GMTs with at least four times of in-creases after vaccination were detected respectively in 53% and 100% of serogroups A and C vaccinated subjects.Pearson correlation coefficient between IgG concentration and rSBA GMTs was r=0.15 (pre) and r=0.23 (post) for serogroup A, and r=-0.14 (pre) and r=0.58 (post) for serogroup C (P<0.05). Conclusion This study has demonstrated an efficient and sustained immunogenicity with meningococcal sero -groups A and C polysaccharide vaccine as evidenced by the data from standardized assays of ELISA and SBA .
