CAJ | 학술논문

目的：通过分析健康人群抗核抗体（ ANA）检测资料，揭示健康人群ANA分布规律，进一步探讨健康人群筛查ANA重要意义。方法采用间接免疫荧光法（ IIF）检测ANA，免疫印迹法检测15项特异性抗体。结果体检人群中，ANA滴度＞1∶100阳性率为14．01％，其中男性阳性率为9．04％，女性阳性率为19．05％；ANA滴度＞1∶320阳性率为5．93％，其中男性阳性率为3．21％，女性阳性率为8．68％；不同性别阳性率差异有统计学意义。 ANA滴度＞1∶320时，男性阳性率随年龄增长呈平缓上升趋势，女性则在青春期及更年期出现两个高峰。 ANA滴度＞1∶320阳性人群15项特异性抗体阳性率44．29％，按出现频率排序前三名是：抗-Ro-52：14．2％；抗-M2：12．7％；抗-SSA：9．6％。结论体检人群各年龄段均存在ANA阳性者，且其分布规律随性别、年龄不同存在明显差异；对于健康人群尤其是处于青春期及更年期的女性筛查ANA是非常必要的，对于筛查阳性人群应做好自身免疫病（ AID）防御知识的宣教及追踪随访工作。
목적：통과분석건강인군항핵항체（ ANA）검측자료，게시건강인군ANA분포규률，진일보탐토건강인군사사ANA중요의의。방법채용간접면역형광법（ IIF）검측ANA，면역인적법검측15항특이성항체。결과체검인군중，ANA적도＞1∶100양성솔위14．01％，기중남성양성솔위9．04％，녀성양성솔위19．05％；ANA적도＞1∶320양성솔위5．93％，기중남성양성솔위3．21％，녀성양성솔위8．68％；불동성별양성솔차이유통계학의의。 ANA적도＞1∶320시，남성양성솔수년령증장정평완상승추세，녀성칙재청춘기급경년기출현량개고봉。 ANA적도＞1∶320양성인군15항특이성항체양성솔44．29％，안출현빈솔배서전삼명시：항-Ro-52：14．2％；항-M2：12．7％；항-SSA：9．6％。결론체검인군각년령단균존재ANA양성자，차기분포규률수성별、년령불동존재명현차이；대우건강인군우기시처우청춘기급경년기적녀성사사ANA시비상필요적，대우사사양성인군응주호자신면역병（ AID）방어지식적선교급추종수방공작。
Objective To study the distribution of antinuclear antibodies ( ANAs) in a healthy population and the significance of using ANAs screening test in medical examination .Methods The ANAs were measured by indirect immunofluorescence assay ( IIF) .The Western blot assay was used to detect fif-teen specific antibodies against auto-antigens .Results 3519 out of all 25 110 subjects showed ANAs titers>1∶100 , and among them male and female subjects were respectively accounted for 1143 and 2376 .1489 out of all subjects had ANAs titers >1∶320 , and among them male and female subjects were respectively accounted for 406 and 1083 .The positive rates of ANAs at different titers showed significant differences be -tween male and female subjects .Among subjects with ANAs titers >1∶320 , the number of male subjects showed a steady increase with the age , while the percentage of female subjects reached to two peaks during the periods of puberty and menopause .The fifteen specific antibodies were detected in 659 out of 1489 sub-jects with ANAs titers>1∶320 and anti-Ro-52 (14.2%) accounted for the majority , followed by anti-M2 (12.7%) and anti-SSA (9.6%).Conclusion ANAs can be detected among healthy population of all ages, but their distribution varied with gender and age .ANAs screening test is necessary for medical exami-nation of healthy population , especially for female during period of puberty or menopause .The population with positive ANAs should be followed-up closely and educated for the prevention of autoimmune diseases .