CAJ | 학술논문

为比较NDV江苏分离株的遗传变异特征，应用RT-PCR扩增出新城疫病毒（NDV）江苏徐州株（JSXZ）、江苏宿州株（JSSZ）、江苏连云港株（JSLYG）和江苏淮安株（JSHA）的融合蛋白F基因的全长核苷酸，将其分别克隆至pMD18- T载体中。将获得的阳性重组质粒进行序列测定后，推导出其氨基酸序列，进行分析和比较。结果表明，所获得的4个分离株F基因完整的开放阅读框长度为1662bp，共编码F蛋白的553个氨基酸；4个毒株与常用疫苗株之间核苷酸序列同源性只有69.3%~80.8%。JSXZ株和JSSZ株F基因的裂解位点为112-RRQK/RRF-117，符合强毒株裂解区氨基酸组成的特征。以1bp~374bp的核苷酸序列绘制系统发育树分析表明，JSXZ和JSSZ株NDV为基因Ⅷ型，JSLYG和JSHA株NDV为基因Ⅸ型。
위비교NDV강소분리주적유전변이특정，응용RT-PCR확증출신성역병독（NDV）강소서주주（JSXZ）、강소숙주주（JSSZ）、강소련운항주（JSLYG）화강소회안주（JSHA）적융합단백F기인적전장핵감산，장기분별극륭지pMD18- T재체중。장획득적양성중조질립진행서렬측정후，추도출기안기산서렬，진행분석화비교。결과표명，소획득적4개분리주F기인완정적개방열독광장도위1662bp，공편마F단백적553개안기산；4개독주여상용역묘주지간핵감산서렬동원성지유69.3%~80.8%。JSXZ주화JSSZ주F기인적렬해위점위112-RRQK/RRF-117，부합강독주렬해구안기산조성적특정。이1bp~374bp적핵감산서렬회제계통발육수분석표명，JSXZ화JSSZ주NDV위기인Ⅷ형，JSLYG화JSHA주NDV위기인Ⅸ형。
In order to understand the features of hereditary variation of NDV isolates from Jiangsu,the fusion glyco-protein(F)genes of NDV Xuzhou(JSXZ),Suzhou(JSSZ),Lianyungang(JSLYG)and Huaian 1(JSHA) isolates were amplified by reverse transcriptase polymerase chain reaction(RT-PCR)and cloned into pMD 18-T vector. The recombinant plasmids were sequenced. Their cDNA were obtained and their amino acid sequences were deduced, analyzed and compared. The results showed that complete open read frame of F gene of the 4 isolates were 1662 bp and encoded 553 amino acids. The nucleotide sequence homologies among the 4 isolates and NDV vaccine strain were 69.3%-80.8%. The deduced amid acid sequence of the cleavage site region of JSXZ and JSSZ F protein was 112-RRQK/RRF-117, conforming to the feature of velogenic strain. Polygenetic tree of NDV strains were constructed by nucleotide acid se-quences of a 374 bp region of F gene and showed that JSXZ and JSSZ isolates belonged to the genetypeⅧ, JSLYG and JSHA isolates belong to the genetypeⅨ.