西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2014年
3期
411-414
,共4页
甄永占%胡刚%赵毓芳%李冉%章广玲%朱丽华%林雅军
甄永佔%鬍剛%趙毓芳%李冉%章廣玲%硃麗華%林雅軍
견영점%호강%조육방%리염%장엄령%주려화%림아군
赖氨大黄酸(RHL)%SAMP 10 快速老化小鼠%肾小球肾炎%肿瘤坏死因子-α%白细胞介素6(IL-6)%核因子κB (NF-κB)%抗衰老
賴氨大黃痠(RHL)%SAMP 10 快速老化小鼠%腎小毬腎炎%腫瘤壞死因子-α%白細胞介素6(IL-6)%覈因子κB (NF-κB)%抗衰老
뢰안대황산(RHL)%SAMP 10 쾌속노화소서%신소구신염%종류배사인자-α%백세포개소6(IL-6)%핵인자κB (NF-κB)%항쇠로
rhein lysinate%senescence accelerated mouse prone 1 0 (SAMP 1 0 )%glomerulonephritis%tumor necrosis factor-alpha (TNF-α)%IL-6%NF-κB%anti-aging
目的：研究赖氨大黄酸(RHL)对快速老化小鼠(SAMP 10)肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)及核因子κB(NF-κB)蛋白表达调控的影响及其意义。方法选取7月龄 SAMP 10小鼠18只,随机分为空白对照组及不同剂量的RHL组,另选抗快速老化小鼠(SAMR 1)6只作为青年对照,给药时间6周。采用 HE染色观察肾脏组织病理学改变,免疫组化和 Western blotting 方法检测肾组织 TNF-α、IL-6、NF-κB 蛋白的表达。结果 RHL治疗对 SAMP10小鼠体重没有显著影响(P>0.05)。与 SAMR 1组比较,SAMP 10小鼠肾组织有节段性肾小球萎缩、硬化和炎细胞浸润,而RHL(25 mg/kg和50 mg/kg)治疗能阻断这一病理进程。另外,RHL治疗能抑制SAMP 10小鼠肾组织TNF-α、IL-6、NF-κB和磷酸化的 NF-κB蛋白过度表达(P<0.05)。结论 RHL可抑制 SAMP 10小鼠肾组织炎症反应,这可能是其发挥肾保护作用,从而起到抗衰老作用的机制之一。
目的：研究賴氨大黃痠(RHL)對快速老化小鼠(SAMP 10)腎組織腫瘤壞死因子-α(TNF-α)、白細胞介素6(IL-6)及覈因子κB(NF-κB)蛋白錶達調控的影響及其意義。方法選取7月齡 SAMP 10小鼠18隻,隨機分為空白對照組及不同劑量的RHL組,另選抗快速老化小鼠(SAMR 1)6隻作為青年對照,給藥時間6週。採用 HE染色觀察腎髒組織病理學改變,免疫組化和 Western blotting 方法檢測腎組織 TNF-α、IL-6、NF-κB 蛋白的錶達。結果 RHL治療對 SAMP10小鼠體重沒有顯著影響(P>0.05)。與 SAMR 1組比較,SAMP 10小鼠腎組織有節段性腎小毬萎縮、硬化和炎細胞浸潤,而RHL(25 mg/kg和50 mg/kg)治療能阻斷這一病理進程。另外,RHL治療能抑製SAMP 10小鼠腎組織TNF-α、IL-6、NF-κB和燐痠化的 NF-κB蛋白過度錶達(P<0.05)。結論 RHL可抑製 SAMP 10小鼠腎組織炎癥反應,這可能是其髮揮腎保護作用,從而起到抗衰老作用的機製之一。
목적：연구뢰안대황산(RHL)대쾌속노화소서(SAMP 10)신조직종류배사인자-α(TNF-α)、백세포개소6(IL-6)급핵인자κB(NF-κB)단백표체조공적영향급기의의。방법선취7월령 SAMP 10소서18지,수궤분위공백대조조급불동제량적RHL조,령선항쾌속노화소서(SAMR 1)6지작위청년대조,급약시간6주。채용 HE염색관찰신장조직병이학개변,면역조화화 Western blotting 방법검측신조직 TNF-α、IL-6、NF-κB 단백적표체。결과 RHL치료대 SAMP10소서체중몰유현저영향(P>0.05)。여 SAMR 1조비교,SAMP 10소서신조직유절단성신소구위축、경화화염세포침윤,이RHL(25 mg/kg화50 mg/kg)치료능조단저일병리진정。령외,RHL치료능억제SAMP 10소서신조직TNF-α、IL-6、NF-κB화린산화적 NF-κB단백과도표체(P<0.05)。결론 RHL가억제 SAMP 10소서신조직염증반응,저가능시기발휘신보호작용,종이기도항쇠로작용적궤제지일。
Objective To investigate the effects of rhein lysinate (RHL)on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of senescence accelerated mouse prone 10 (SAMP 10)mice.Methods We selected 1 8 male mice (SAMP 1 0 )aged 7 months for the study and randomly divided them into blank control group and groups of different concentrations of RHL;six senescence accelerated mouse resistance 1 (SAMR 1 )served as the young control group.After 6 weeks’ treatment,HE staining was used to detect the pathological changes of the kidney.The expressions of TNF-α,IL-6 and NF-κB at the protein level were detected by immunohistochemistry and Western blotting.Results RHL treatment did not affect the body weight of SAMP 10 mice (P>0.05 ). Compared with SAMR 1 mice, contracted and destroyed renal glomeruli and infiltration of mononuclear macrophages were observed in control SAMP10 mice.However,this pathological process was blocked by RHL (25 mg/kg and 50 mg/kg ) treatments. In addition, the overexpressions of TNF-α, IL-6 and NF-κB and the phosphorylation of NF-κB in the kidney tissue of SAMP 10 mice could be inhibited by RHL treatments (P<0.05). Conclusion RHL inhibits the inflammatory reaction of the kidney tissue,which may be one of the mechanisms by which RHL exerts its kidney-protecting and anti-aging effects.
