CAJ | 학술논문

目的：探讨上皮间质转化(EMT)是否参与姜黄素抑制乳腺癌细胞的侵袭转移过程及其可能的机制。方法利用不同浓度姜黄素干预脂多糖(LPS)诱导的乳腺癌细胞 MDA-MB-231,MTT 法检测细胞增殖能力,普通光镜及透射电镜观察细胞形态变化,RT-PCR 和 Western blotting 方法分别检测 Vimentin、E-cadherin、转录因子 NF-κB、Snail 表达变化。结果姜黄素可抑制 NF-κB-Snail信号轴活化来抑制 LPS诱导的乳腺癌细胞 MDA-MB-231 EMT 的发生,降低 LPS诱导的 EMT标志物 Vimentin蛋白的表达,增强 E-cadherin的表达,最终导致乳腺癌细胞运动及侵袭能力的降低。结论本研究为姜黄素的抗肿瘤侵袭能力提供了新视角,提示其抑制侵袭转移能力可能是通过抑制 E MT程序来发挥作用的。
목적：탐토상피간질전화(EMT)시부삼여강황소억제유선암세포적침습전이과정급기가능적궤제。방법이용불동농도강황소간예지다당(LPS)유도적유선암세포 MDA-MB-231,MTT 법검측세포증식능력,보통광경급투사전경관찰세포형태변화,RT-PCR 화 Western blotting 방법분별검측 Vimentin、E-cadherin、전록인자 NF-κB、Snail 표체변화。결과강황소가억제 NF-κB-Snail신호축활화래억제 LPS유도적유선암세포 MDA-MB-231 EMT 적발생,강저 LPS유도적 EMT표지물 Vimentin단백적표체,증강 E-cadherin적표체,최종도치유선암세포운동급침습능력적강저。결론본연구위강황소적항종류침습능력제공료신시각,제시기억제침습전이능력가능시통과억제 E MT정서래발휘작용적。
Objective To investigate whether epithelial-mesenchymal transition (EMT)is involved in the anti-invasion and anti-metastasis effects of curcumin on MDA-MB-2 3 1 breast cancer cell line and further analyze the underlying mechanisms.Methods MTT method was used to detect the anti-proliferative effect of curcumin on MDA-MB-2 3 1 cell line induced by lipopolysaccharide (LPS).The morphological changes were determined by optical and transmission electron microscopy,respectively.The expressions of Vimentin,E-cadherin,NF-κB and Snail were analyzed using RT-PCR and Western blotting.Results Curcumin could inhibit the occurrence of LPS-induced EMT of MDA-MB-2 3 1 breast cancer cell line, decrease the expression of LPS-induced EMT marker Vimentin and increase the expression of E-cadherin, resulting in the inhibition of in vitro cell motility and invasiveness.These actions were mediated by inactivating NF-κB-Snail signaling pathways.Conclusion Our data provide a new perspective of the anti-invasion mechanism of curcumin,indicating that the effect is in part due to its ability to inhibit the EMT process.