CAJ | 학술논문

目的： CST6基因编码 cystatin M蛋白，体外构建CST6过表达载体，转染胃癌 SGC-7901细胞株，观察 cystatin M对其增殖和迁移能力的影响。方法实验分3组：pcDNA3.1(+)-CST6组、pcDNA3.1(+)组与未转染组；RT-PCR验证稳转系 SGC-7901/CST6细胞 CST6 RNA 水平的表达；Western blot 验证稳转系 SGC-7901/CST6细胞CST6蛋白水平的表达；MTT实验检测实验组与对照组细胞的增殖能力；划痕实验检测实验组与对照组细胞的迁移能力。结果稳转系 SGC-7901/CST6细胞稳定表达CST6基因mRNA和 cystatin M蛋白；pcDNA3.1(+)-CST6组细胞增殖能力和迁移能力均较 pcDNA3.1(+)组和未转染组细胞降低(P<0.05)。结论 Cystatin M能够抑制胃癌SGC-7901细胞株增殖和迁移能力。
목적： CST6기인편마 cystatin M단백，체외구건CST6과표체재체，전염위암 SGC-7901세포주，관찰 cystatin M대기증식화천이능력적영향。방법실험분3조：pcDNA3.1(+)-CST6조、pcDNA3.1(+)조여미전염조；RT-PCR험증은전계 SGC-7901/CST6세포 CST6 RNA 수평적표체；Western blot 험증은전계 SGC-7901/CST6세포CST6단백수평적표체；MTT실험검측실험조여대조조세포적증식능력；화흔실험검측실험조여대조조세포적천이능력。결과은전계 SGC-7901/CST6세포은정표체CST6기인mRNA화 cystatin M단백；pcDNA3.1(+)-CST6조세포증식능력화천이능력균교 pcDNA3.1(+)조화미전염조세포강저(P<0.05)。결론 Cystatin M능구억제위암SGC-7901세포주증식화천이능력。
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.