CAJ | 학술논문

目的：研究慢性乙型肝炎患者和慢加急性乙型肝炎肝衰竭（HBV-ACLF）患者外周血单个核细胞（PBMC）Toll 样受体2（TLR2）表达，以及鼠三型肝炎病毒（MHV-3）诱导的暴发性肝炎小鼠肝脏 TLR2表达的变化。方法收集慢性乙型肝炎和 HBV-ACLF 患者外周血，分离 PBMC，采用实时定量 PCR 法检测 PBMC 中 TLR2 mRNA；给 Balb/cJ 小鼠腹腔注射MHV-3（100 pfu），建立小鼠暴发性肝炎模型，观察感染0、24、48和72 h 后肝脏TLR2水平变化。结果 BALB/cJ 小鼠在感染 MHV-3后，与0 h[（0.39±0.06）%]比，肝细胞 TLR2 mRNA 水平在感染48和72 h 均显著升高[分别为（9.06±1.60）%和（6.42±2.42）%，P＜0.05）]，并于48 h 达最高水平，且两时间点细胞 TLR2 mRNA 水平均与血清 ALT 和 AST 水平呈正相关（r=0.804，P＜0.01；r=0.797，P＜0.01）；HBV-ACLF患者 PBMC 中 TLR2 mRNA 水平显著高于慢性乙型肝炎患者[（5.92±5.26）%对（1.15±1.59）%，P＜0.05）]。结论 TLR2参与了 MHV-3诱导的暴发性肝炎小鼠以及 HBV-ACLF 患者肝脏损伤的发病过程。
목적：연구만성을형간염환자화만가급성을형간염간쇠갈（HBV-ACLF）환자외주혈단개핵세포（PBMC）Toll 양수체2（TLR2）표체，이급서삼형간염병독（MHV-3）유도적폭발성간염소서간장 TLR2표체적변화。방법수집만성을형간염화 HBV-ACLF 환자외주혈，분리 PBMC，채용실시정량 PCR 법검측 PBMC 중 TLR2 mRNA；급 Balb/cJ 소서복강주사MHV-3（100 pfu），건립소서폭발성간염모형，관찰감염0、24、48화72 h 후간장TLR2수평변화。결과 BALB/cJ 소서재감염 MHV-3후，여0 h[（0.39±0.06）%]비，간세포 TLR2 mRNA 수평재감염48화72 h 균현저승고[분별위（9.06±1.60）%화（6.42±2.42）%，P＜0.05）]，병우48 h 체최고수평，차량시간점세포 TLR2 mRNA 수평균여혈청 ALT 화 AST 수평정정상관（r=0.804，P＜0.01；r=0.797，P＜0.01）；HBV-ACLF환자 PBMC 중 TLR2 mRNA 수평현저고우만성을형간염환자[（5.92±5.26）%대（1.15±1.59）%，P＜0.05）]。결론 TLR2삼여료 MHV-3유도적폭발성간염소서이급 HBV-ACLF 환자간장손상적발병과정。
Objective To investigate the toll-like receptor 2 (TLR2) in livers of mice with murine hepati-tis virus-3 (MHV-3)-induced fulminant hepatitis and in peripheral blood mononuclear cell (PBMCs) from pa-tients with mild chronic hepatitis B (CHB)or with HBV-associated acute-on-chronic liver failure (HBV-ACLF). Methods PBMCs from patients with mild CHB and HBV-ACLF were collected. The TLR2 mRNA in PBMCs were determined by quantitative real-time PCR. Balb/cJ mice were injected intraperitoneally with MHV-3 at dose of 100 pfu to induce fulminant hepatitis. At 0,24,48 and 72 h after injection,the TLR2 mRNA in liver tissues of mice were determined. Results After infection of MHV-3,TLR2 mRNA increased significantly at 48 and 72 h when compared with the baseline at 0 h [(0.39±0.06)% vs.(9.06±1.60)%,P<0.05 and(6.42±2.42)%,P<0.05] and it peaked at 48 h;mRNA levels for TLR2 at 48 and 72 h were positively correlated with the serum ALT and AST(r=0.804,P<0.01;r=0.797,P<0.01,respectively);mRNA level of TLR2 in PBMCs from patients with HBV-A-CLF was significantly higher than patients with mild CHB [(5.92±5.26)% vs.(1.15±1.59)%,P<0.05]. Conclu-sions TLR2 may take part in the process of liver injury in MHV-3-induced fulminant hepatitis in mice and the pathogenesis of patients with HBV-ACLF.