西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2014年
3期
338-341,351
,共5页
角膜新生血管%血管内皮生长因子%胰岛素样生长因子-1%奥曲肽
角膜新生血管%血管內皮生長因子%胰島素樣生長因子-1%奧麯肽
각막신생혈관%혈관내피생장인자%이도소양생장인자-1%오곡태
corneal neovascularization (CNV )%vascular endothelial growth factor (VEGF )%insulin like growth factor-1 (IGF-1 )%octreotide
目的：研究奥曲肽(octreotide)对角膜新生血管(corneal neovascularization,CNV)形成的影响。方法18只成年雌性健康 SD大鼠,左眼为对照组,右眼为奥曲肽组,每组再分4、7、14 d共3个时间点,每个时间点6只眼。所有大鼠双眼均通过角膜缝线法建立CNV模型,对照组术后给予生理盐水滴眼,奥曲肽组术后给予0.1 mg/mL 奥曲肽滴眼。显微镜下观察新生血管生长情况,免疫组织化学染色检测各时间点角膜组织中血管内皮生长因子(vascular en-dothelial growth factor,VEGF)和胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)的表达变化。结果奥曲肽组术后4、7、14 d CNV 面积均小于对照组(P<0.05),VEGF 蛋白和 IGF-1蛋白表达量低于对照组(P<0.05)。结论奥曲肽能下调缝线后角膜组织 VEGF和 IGF-1蛋白的表达,抑制CNV形成。
目的：研究奧麯肽(octreotide)對角膜新生血管(corneal neovascularization,CNV)形成的影響。方法18隻成年雌性健康 SD大鼠,左眼為對照組,右眼為奧麯肽組,每組再分4、7、14 d共3箇時間點,每箇時間點6隻眼。所有大鼠雙眼均通過角膜縫線法建立CNV模型,對照組術後給予生理鹽水滴眼,奧麯肽組術後給予0.1 mg/mL 奧麯肽滴眼。顯微鏡下觀察新生血管生長情況,免疫組織化學染色檢測各時間點角膜組織中血管內皮生長因子(vascular en-dothelial growth factor,VEGF)和胰島素樣生長因子-1(insulin-like growth factor-1,IGF-1)的錶達變化。結果奧麯肽組術後4、7、14 d CNV 麵積均小于對照組(P<0.05),VEGF 蛋白和 IGF-1蛋白錶達量低于對照組(P<0.05)。結論奧麯肽能下調縫線後角膜組織 VEGF和 IGF-1蛋白的錶達,抑製CNV形成。
목적：연구오곡태(octreotide)대각막신생혈관(corneal neovascularization,CNV)형성적영향。방법18지성년자성건강 SD대서,좌안위대조조,우안위오곡태조,매조재분4、7、14 d공3개시간점,매개시간점6지안。소유대서쌍안균통과각막봉선법건립CNV모형,대조조술후급여생리염수적안,오곡태조술후급여0.1 mg/mL 오곡태적안。현미경하관찰신생혈관생장정황,면역조직화학염색검측각시간점각막조직중혈관내피생장인자(vascular en-dothelial growth factor,VEGF)화이도소양생장인자-1(insulin-like growth factor-1,IGF-1)적표체변화。결과오곡태조술후4、7、14 d CNV 면적균소우대조조(P<0.05),VEGF 단백화 IGF-1단백표체량저우대조조(P<0.05)。결론오곡태능하조봉선후각막조직 VEGF화 IGF-1단백적표체,억제CNV형성。
Objective To observe the effect of octreotide on corneal neovascularization (CNV).Methods Totally 1 8 healthy adult SD rats were randomly divided into octreotide group and control group.Each group was further divided into 3 time points:4 d,7 d and 1 4 d.At each time point 6 eyes were used.All the rats were given corneal suture to induce CNV.Control group was given normal saline eye drops and octreotide group was given 0.1 mg/mL octreotide solution eye drops after surgery,4 times daily.The occurrence and development of CNV were observed under the microscope and the expressions of vascular endothelial growth factor (VEGF)and insulin like growth factor-1 (IGF-1)proteins at each time point were detected by immunohistochemical staining.Results CNV area at postsurgical day 4,7 and 14 was significantly smaller in octreotide group than in control group (P<0 .0 5 ).Expressions of VEGF and IGF-1 proteins were obviously lower in octreotide group than in control group (P<0.05)at each time point.Conclusion Octreotide can inhibit CNV effectively after corneal suture through down-regulating the expressions of VEGF and IGF-1 in the cornea.
