中国医药导刊
中國醫藥導刊
중국의약도간
CHINESE JOURNAL OF MEDICAL GUIDE
2014年
4期
647-648,650
,共3页
马传胜%周传江%王运锋%赵威
馬傳勝%週傳江%王運鋒%趙威
마전성%주전강%왕운봉%조위
FEZ1基因%单核苷酸多态性%食管鳞癌
FEZ1基因%單覈苷痠多態性%食管鱗癌
FEZ1기인%단핵감산다태성%식관린암
FEZ1 gene%Single nucleotide polymorphism%Esophageal squamous cell carcinoma
目的：研究FEZ1(fasciculation and elongation protein zeta 1)基因外显子区rs559668多态性表达与食管鳞状细胞癌(Esophageal squamous cell carcinoma ESCC)临床指标及恶性程度的相关性。方法：(1)提取90例食管鳞癌手术切除癌组织标本和正常食管组织标本28例,同时评估鳞癌分化程度及进行TNM分期。(2)应用PCR-RFLP技术扩增FEZ1基因,并对目的基因进行酶切,测定FEZ1基因rs559668的多态性,并进行基因分型,进行不同基因型与临床病理因素的相关性研究。结果：鳞癌组织中C等位基因的频率显著低于正常组织(41.11%vs 67.86%,P<0.05)；鳞癌组织中CC基因型频率显著低于正常组织(24.44%vs 42.86%, P<0.05)；与CC及TC基因型组相比,TT基因型组分化程度低和浸润程度深TNM分期较晚。结论：FEZ1基因rs559668的C等位基因与食管鳞癌的发生具有相关性,与鳞癌的分化程度、浸润深度、转移范围以及TNM分期呈负相关。因此FEZ1基因可视为一种抑癌基因。
目的：研究FEZ1(fasciculation and elongation protein zeta 1)基因外顯子區rs559668多態性錶達與食管鱗狀細胞癌(Esophageal squamous cell carcinoma ESCC)臨床指標及噁性程度的相關性。方法：(1)提取90例食管鱗癌手術切除癌組織標本和正常食管組織標本28例,同時評估鱗癌分化程度及進行TNM分期。(2)應用PCR-RFLP技術擴增FEZ1基因,併對目的基因進行酶切,測定FEZ1基因rs559668的多態性,併進行基因分型,進行不同基因型與臨床病理因素的相關性研究。結果：鱗癌組織中C等位基因的頻率顯著低于正常組織(41.11%vs 67.86%,P<0.05)；鱗癌組織中CC基因型頻率顯著低于正常組織(24.44%vs 42.86%, P<0.05)；與CC及TC基因型組相比,TT基因型組分化程度低和浸潤程度深TNM分期較晚。結論：FEZ1基因rs559668的C等位基因與食管鱗癌的髮生具有相關性,與鱗癌的分化程度、浸潤深度、轉移範圍以及TNM分期呈負相關。因此FEZ1基因可視為一種抑癌基因。
목적：연구FEZ1(fasciculation and elongation protein zeta 1)기인외현자구rs559668다태성표체여식관린상세포암(Esophageal squamous cell carcinoma ESCC)림상지표급악성정도적상관성。방법：(1)제취90례식관린암수술절제암조직표본화정상식관조직표본28례,동시평고린암분화정도급진행TNM분기。(2)응용PCR-RFLP기술확증FEZ1기인,병대목적기인진행매절,측정FEZ1기인rs559668적다태성,병진행기인분형,진행불동기인형여림상병리인소적상관성연구。결과：린암조직중C등위기인적빈솔현저저우정상조직(41.11%vs 67.86%,P<0.05)；린암조직중CC기인형빈솔현저저우정상조직(24.44%vs 42.86%, P<0.05)；여CC급TC기인형조상비,TT기인형조분화정도저화침윤정도심TNM분기교만。결론：FEZ1기인rs559668적C등위기인여식관린암적발생구유상관성,여린암적분화정도、침윤심도、전이범위이급TNM분기정부상관。인차FEZ1기인가시위일충억암기인。
Objective: To investigate the relationship between the T/C genotypes of fasciculation and elongation protein zeta 1(FEZ1)single nucleotide polymorphism(SNP) at rs559668 and the development and progression of Esophageal squamous cell carcinoma(ESCC). Methods:There were 118excision tissues, including 90ESCC and 28 corresponding normal tissues, were determined by clinical patholosical analysis. Polymerase chain reaction(PCR)and restriction fragment length polymorphism(RFLP)were used to determine a common polymorphism of the human FEZ1 gene, rs559668 T>C in 90 ESCC patients and 28 corresponding normal tissues. <br> Results:The frequency for C allelic genes was significant lower in squamous carcinoma than in controls(41.11%vs 67.86%, p<0.05). The CC genotypes were significant lower in squamous carcinoma than in controls(24.44%vs 42.86%, p<0.05). The TT genotypes to rs559668 of FEZ1 gene are sensitive to the progression of ESCC. Conclusion:The T/C homozygotes of FEZ1 single nucleotide at rs559668 are assiociated with ESCC patients. The TT genotypes to rs559668 of FEZ1 gene are sensitive to the progression of ESCC. 2. The expression rates of FEZ1 is also related to the depth of tumor invasion and differentiated group and lymphnode involvement and TNM staging.
