CAJ | 학술논문

嗜酸浸矿微生物产生的胞外多聚物(EPS)在酸性矿坑水的产生和硫化矿的浸出过程中，有着非常重要的影响，胞外多聚物(EPS)介导细胞与能源物质的接触，对有机薄膜的形成和细菌与基础物之间的相互作用起着重要的作用。对7株浸矿菌在不同能源培养物下产生的EPS的量以及EPS的化学成分进行研究，发现EPS含有化学成分糖、蛋白质、糖醛酸等，细菌的种类和能源物质对EPS的量和成分有很大影响。结果表明，以黄铜矿为能源物质的细菌产生的EPS要比以单质硫和亚铁为能源物质产生的EPS量多，EPS含量最高为(159.43±3.93) mg/g，是由Leptospirillum ferriphilum CBCBSUCSU208015在黄铁矿为能源物质下产生的。
기산침광미생물산생적포외다취물(EPS)재산성광갱수적산생화류화광적침출과정중，유착비상중요적영향，포외다취물(EPS)개도세포여능원물질적접촉，대유궤박막적형성화세균여기출물지간적상호작용기착중요적작용。대7주침광균재불동능원배양물하산생적EPS적량이급EPS적화학성분진행연구，발현EPS함유화학성분당、단백질、당철산등，세균적충류화능원물질대EPS적량화성분유흔대영향。결과표명，이황동광위능원물질적세균산생적EPS요비이단질류화아철위능원물질산생적EPS량다，EPS함량최고위(159.43±3.93) mg/g，시유Leptospirillum ferriphilum CBCBSUCSU208015재황철광위능원물질하산생적。
Extracellular polymeric substances (EPS) produced by acidophilic bioleaching microorganisms play an important role in the production of acid mine drainage and metal sulfide bioleaching. EPS mediate the contact between microbial cells and growth substrates, having a pivotal role in organic film formation and bacterium-substratum interactions. The production and chemical composition of EPS produced by seven bioleaching strains grown with different substrates were studied. Analysis of the EPS extracted from these strains indicated that the EPS consisted of carbohydrates, proteins and galacturonic acid. The contents of EPS, carbohydrates, proteins and galacturonic acid of EPS were largely related to the kind of strain used and culture condition. The results show that EPS productions of microbes grown with pyrite were significantly higher than those of microbes grown with sulfur or FeSO4·7H2O. The highest EPS production of the seven acidiphilic strains was (159.43±3.93) mg/g, which was produced by Leptospirillum ferriphilum CBCBSUCSU208015 when cultivated with pyrite.