中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
703-705
,共3页
高效液相色谱法%黄芩苷%虎杖苷%抗感利咽糖浆%含量测定
高效液相色譜法%黃芩苷%虎杖苷%抗感利嚥糖漿%含量測定
고효액상색보법%황금감%호장감%항감리인당장%함량측정
HPLC%Baicalin%Polydatin%Kanggan Liyan syrups%Determination
目的：建立一种同时测定抗感利咽糖浆中黄芩苷、虎杖苷含量的高效液相色谱法。方法：采用Waters SunFire C18色谱柱(250 mm ×4.6 mm,5μm)；乙腈为流动相A,0.2%磷酸溶液为流动相B,梯度洗脱,流速为1.0 ml·min-1,检测波长为284 nm,柱温为30℃。结果：黄芩苷、虎杖苷与其他成分分离度良好,无干扰；黄芩苷在32.0~480.0μg·ml-1的范围内有良好的线性关系,平均回收率为98.71%,RSD为0.67%；虎杖苷在16.0~240.0μg·ml-1范围内有良好线性关系,平均回收率为97.02%,RSD为1.03%。结论：所建立HPLC法可同时测定抗感利咽糖浆中黄芩苷和虎杖苷的含量,方法准确度高,稳定性好,可作为该制剂的含量测定方法。
目的：建立一種同時測定抗感利嚥糖漿中黃芩苷、虎杖苷含量的高效液相色譜法。方法：採用Waters SunFire C18色譜柱(250 mm ×4.6 mm,5μm)；乙腈為流動相A,0.2%燐痠溶液為流動相B,梯度洗脫,流速為1.0 ml·min-1,檢測波長為284 nm,柱溫為30℃。結果：黃芩苷、虎杖苷與其他成分分離度良好,無榦擾；黃芩苷在32.0~480.0μg·ml-1的範圍內有良好的線性關繫,平均迴收率為98.71%,RSD為0.67%；虎杖苷在16.0~240.0μg·ml-1範圍內有良好線性關繫,平均迴收率為97.02%,RSD為1.03%。結論：所建立HPLC法可同時測定抗感利嚥糖漿中黃芩苷和虎杖苷的含量,方法準確度高,穩定性好,可作為該製劑的含量測定方法。
목적：건립일충동시측정항감리인당장중황금감、호장감함량적고효액상색보법。방법：채용Waters SunFire C18색보주(250 mm ×4.6 mm,5μm)；을정위류동상A,0.2%린산용액위류동상B,제도세탈,류속위1.0 ml·min-1,검측파장위284 nm,주온위30℃。결과：황금감、호장감여기타성분분리도량호,무간우；황금감재32.0~480.0μg·ml-1적범위내유량호적선성관계,평균회수솔위98.71%,RSD위0.67%；호장감재16.0~240.0μg·ml-1범위내유량호선성관계,평균회수솔위97.02%,RSD위1.03%。결론：소건립HPLC법가동시측정항감리인당장중황금감화호장감적함량,방법준학도고,은정성호,가작위해제제적함량측정방법。
Objective:To establish an HPLC method for the determination of baicalin and polydatin in Kanggan Liyan syrups. Methods:The samples were analyzed on an Waters SunFire C18 column with the mobile phase A of acetonitrile and the mobile phase B of 0. 2% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 284nm,and the column box temperature was 30℃. Results:Baicalin and polydatin could be separated effectively without interference. The linear range of baicalin was 32. 0-480. 0 μg·ml-1 and the average recovery was 98. 71%(RSD=0. 67%,n=5). The linear range of poly-datin was 16. 0-240. 0 μg·ml-1 and the average recovery was 97. 02%(RSD=1. 03%,n=5). Conclusion:The method is accurate and stable, and can be used in the determination of Kanggan Liyan syrups.
