CAJ | 학술논문

目的：优选大孔树脂分离木瓜中总黄酮的工艺条件。方法：以木瓜中总黄酮含量为指标,通过单因素试验考察树脂型号、上样液质量浓度、树脂柱径高比、吸附流速、洗脱剂种类及用量、除杂溶媒种类及用量、洗脱流速等工艺参数对纯化工艺的影响。结果：采用D-140型大孔树脂,优选的纯化工艺为上样液质量浓度为0.1 g·ml-1,上样量为2 BV,径高比1∶9,用3 BV水洗除杂,3 BV10%乙醇洗脱,3 BV50%乙醇以2 BV·h-1洗脱,收集50%乙醇洗脱液,总黄酮质量分数达52%。结论：优选的工艺条件可较好的分离、纯化木瓜中总黄酮。
목적：우선대공수지분리목과중총황동적공예조건。방법：이목과중총황동함량위지표,통과단인소시험고찰수지형호、상양액질량농도、수지주경고비、흡부류속、세탈제충류급용량、제잡용매충류급용량、세탈류속등공예삼수대순화공예적영향。결과：채용D-140형대공수지,우선적순화공예위상양액질량농도위0.1 g·ml-1,상양량위2 BV,경고비1∶9,용3 BV수세제잡,3 BV10%을순세탈,3 BV50%을순이2 BV·h-1세탈,수집50%을순세탈액,총황동질량분수체52%。결론：우선적공예조건가교호적분리、순화목과중총황동。
Objective:To optimize the macroporous resin separation process for total flavonoids in papaya. Methods:The content of total flavonoids in papaya was selected as the index, and the resin model, sample solution concentration, ratio of diameter and height, the flow rate of adsorption, type and volume of eluent, type and volume of impurity removing solvent, elution velocity and the other parameters were investigated. Results:The optimal purification process was as follows: the macroporous resin type was D-140, the sample solution concentration was 0. 1 g·ml-1 , the sample volume was 2BV, the ratio of diameter and height was 1∶9, washing the impurities with 3BV water, eluting with 3BV 10% ethanol first followed by 3BV 50% ethanol with 2BV·h-1 , and collecting 50%ethanol elution. The total flavonoids content was 52%. Conclusion:The optimized process can separate and purify the total flavonoids in papaya effectively.