中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
540-543
,共4页
脂联素%基因多态性%外显子2%2型糖尿病
脂聯素%基因多態性%外顯子2%2型糖尿病
지련소%기인다태성%외현자2%2형당뇨병
Adiponectin%Polymorphism%Exon 2%Type 2 diabetes mellitus
目的：研究脂联素基因外显子2单核苷酸多态性(SNP)+45T→G多态性与广西地区汉族2型糖尿病(T2DM)、肥胖及胰岛素抵抗( IR)的关系。方法：运用聚合酶链反应-限制性片段长度多态性( PCR-RFLP)的方法,对313例无亲缘关系的广西地区汉族人群的正常糖耐量(NGT95例)、糖耐量减低(IGT103例)和T2DM115例检测脂联素基因外显子2的多态性,并与代谢值进行相关性研究。结果：广西地区汉族人群中含脂联素基因外显子2的SNP+45GG基因型的人群高密度脂蛋白( HDL-C)水平低于TT、TG基因型的人群(P<0.05),HOMA-IR高组的GG基因型相对于TT基因型的OR为2.876(95%CI：1.141~7.248),即GG基因型发生IR的相对危险度是TT基因型的2.876倍。结论：汉族人群中,脂联素基因SNPs+45 TT基因型的HDL-C较GG基因型频率高；GG基因型较TT基因型人群易产生IR。但汉族人群SNPs+45基因型与T2DM及肥胖无关。
目的：研究脂聯素基因外顯子2單覈苷痠多態性(SNP)+45T→G多態性與廣西地區漢族2型糖尿病(T2DM)、肥胖及胰島素牴抗( IR)的關繫。方法：運用聚閤酶鏈反應-限製性片段長度多態性( PCR-RFLP)的方法,對313例無親緣關繫的廣西地區漢族人群的正常糖耐量(NGT95例)、糖耐量減低(IGT103例)和T2DM115例檢測脂聯素基因外顯子2的多態性,併與代謝值進行相關性研究。結果：廣西地區漢族人群中含脂聯素基因外顯子2的SNP+45GG基因型的人群高密度脂蛋白( HDL-C)水平低于TT、TG基因型的人群(P<0.05),HOMA-IR高組的GG基因型相對于TT基因型的OR為2.876(95%CI：1.141~7.248),即GG基因型髮生IR的相對危險度是TT基因型的2.876倍。結論：漢族人群中,脂聯素基因SNPs+45 TT基因型的HDL-C較GG基因型頻率高；GG基因型較TT基因型人群易產生IR。但漢族人群SNPs+45基因型與T2DM及肥胖無關。
목적：연구지련소기인외현자2단핵감산다태성(SNP)+45T→G다태성여엄서지구한족2형당뇨병(T2DM)、비반급이도소저항( IR)적관계。방법：운용취합매련반응-한제성편단장도다태성( PCR-RFLP)적방법,대313례무친연관계적엄서지구한족인군적정상당내량(NGT95례)、당내량감저(IGT103례)화T2DM115례검측지련소기인외현자2적다태성,병여대사치진행상관성연구。결과：엄서지구한족인군중함지련소기인외현자2적SNP+45GG기인형적인군고밀도지단백( HDL-C)수평저우TT、TG기인형적인군(P<0.05),HOMA-IR고조적GG기인형상대우TT기인형적OR위2.876(95%CI：1.141~7.248),즉GG기인형발생IR적상대위험도시TT기인형적2.876배。결론：한족인군중,지련소기인SNPs+45 TT기인형적HDL-C교GG기인형빈솔고；GG기인형교TT기인형인군역산생IR。단한족인군SNPs+45기인형여T2DM급비반무관。
Objective:To evaluate the relationship between single nucleotide polymorphism (SNP) +45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus (T2DM), obesity and insulin resistance (IR) in Han population in Guangxi. Methods:Polymer-ase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) was performed to identify the T-G polymorphism of adiponec-tin (exon 2) in 313 unrelated Han people from Guangxi including 95 normal glucose tolerance,103 impaired glucose tolerance and 115 type 2 diabetes mellitus. Results:In Han people of Guangxi, the SNP +45 GG genotype in adiponectin gene had significantly lower HDL-C levels than TT and TG genotypes in Han people(P<0. 05). The subjects with the GG genotype at position 45 had a signifi-cantly increased risk of IR (odds ratio of 2. 876[95%CI:1. 141-7. 248]) compared with those with the TT genotype at position 45. Conclusion:In Han people of Guangxi, the SNP +45 TT genotype in adiponectin gene has significantly higher HDL-C levels than GG genotype. The subjects with the GG genotype at position 45 have a significantly increased risk of IR compared with those with the TT genotype, while obesity and T2DM are independent on SNP+45 of adiponectin gene.
