CAJ | 학술논문

背景与目的：既往研究表明微小RNA-486-5p(miR-486-5p)在多种肿瘤的进展中起重要作用，但其在胃癌中作用的研究较少，本研究旨在探讨miR-486-5p对胃癌细胞株SGC7901增殖、凋亡及迁移能力的影响。方法：使用实时定量PCR(quantification real-time PCR，qRT-PCR)检测胃癌细胞株SGC7901及胃黏膜上皮细胞GES-1中miR-486-5p的表达，构建miR-486-5p过表达质粒，使用脂质体法瞬时转染胃癌细胞株SGC7901， qRT-PCR检测转染细胞后miR-486-5p的表达丰度，噻唑蓝(MTT)法及流式细胞仪检测细胞的增殖及凋亡情况， Transwell小室迁移实验检测细胞的迁移能力。结果：miR-486-5p在SGC7901细胞中表达明显下调，SGC7901细胞转染miR-486-5p过表达质粒后，miR-486-5p表达明显上调，细胞增殖、迁移能力降低，凋亡率增高，差异均有统计学意义(P<0.05)。结论：miR-486-5p可抑制胃癌细胞株SGC7901的增殖和迁移。
배경여목적：기왕연구표명미소RNA-486-5p(miR-486-5p)재다충종류적진전중기중요작용，단기재위암중작용적연구교소，본연구지재탐토miR-486-5p대위암세포주SGC7901증식、조망급천이능력적영향。방법：사용실시정량PCR(quantification real-time PCR，qRT-PCR)검측위암세포주SGC7901급위점막상피세포GES-1중miR-486-5p적표체，구건miR-486-5p과표체질립，사용지질체법순시전염위암세포주SGC7901， qRT-PCR검측전염세포후miR-486-5p적표체봉도，새서람(MTT)법급류식세포의검측세포적증식급조망정황， Transwell소실천이실험검측세포적천이능력。결과：miR-486-5p재SGC7901세포중표체명현하조，SGC7901세포전염miR-486-5p과표체질립후，miR-486-5p표체명현상조，세포증식、천이능력강저，조망솔증고，차이균유통계학의의(P<0.05)。결론：miR-486-5p가억제위암세포주SGC7901적증식화천이。
Background and purpose:MicroRNA-486-5p (miR-486-5p) has been demonstrated to play an important role in many kinds of tumor, however, there are few reports about the relationship between miRNA-486-5p in gastric carcinoma. This study was aimed to explore the effect of miR-486-5p on the proliferation, apoptosis and migration abilities of the human gastric cancer cell line SGC7901.Methods:Quantitative real-time PCR (qRT-PCR) analysis was performed to detect the expression of miR-486-5p in the SGC7901 and GES-1 cells, miR-486-5p over-expressing plasmid was constructed and transfected into the human gastric carcinoma cell line SGC7901 using LipofectamineTM2000. The expression of miR-486-5p of the transfected cells was measured by qRT-PCR, the proliferation level of SGC7901 cells was detected by MTT method, the apoptosis rate of the cells was measured by lfow cytometry and the in vitro migration abilities of SGC7901 cells by transwell test. Results:The miR-486-5p expression in SGC7901 cells was down-regulated compared with GES-1 cells. The expression of miR-486-5p in SGC7901 cells that was transfected miR-486-5p over-expressing plasmid was obviously up-regulated. The proliferation and migration abilities of SGC7901 cells were inhibited signiifcantly, and the apoptosis rate of the cells increased. Conclusion:miR-486-5p can effectively suppress the proliferation and in vitro migration abilities of SGC7901 cells, indicating that miR-486-5p might be used as a target for molecular therapy of gastric cancer.