医学研究生学报
醫學研究生學報
의학연구생학보
JOURNAL OF MEDICAL POSTGRADUATE
2014年
4期
382-386
,共5页
噬血细胞性淋巴组织细胞增生症%儿童%差异表达基因%通路富集分析
噬血細胞性淋巴組織細胞增生癥%兒童%差異錶達基因%通路富集分析
서혈세포성림파조직세포증생증%인동%차이표체기인%통로부집분석
Hemophagocytic lymphohistiocytosis%Child%Differentially expressed genes%Pathway enrichment analysis
目的：噬血细胞性淋巴组织细胞增生症（ hemophagocytic lymphohistiocytosis ， HLH）是一种致命的过度炎症性疾病，好发于儿童，死亡率高达40％。为进一步了解儿童HLH的发病机制，研究利用生物信息学方法筛选儿童HLH相关基因，并进行通路富集分析。方法从GEO数据库中获得儿童HLH 外周血单个核细胞基因表达谱数据集GSE26050，利用GEO2R在线分析工具筛选差异表达基因，随后使用DAVID数据库的KEGG通路富集方法对其进行分析。结果筛选出表达差异2倍及以上的基因184个，其中126个为上调基因、58个为下调基因；富集出3条通路：细胞因子-细胞因子受体相互作用、造血细胞谱及NOD样受体通路。结论通过生物信息学方法可筛选出儿童HLH相关基因及与之发生发展密切相关的生物通路，为进一步揭示儿童HLH发病机制提供实验参考。
目的：噬血細胞性淋巴組織細胞增生癥（ hemophagocytic lymphohistiocytosis ， HLH）是一種緻命的過度炎癥性疾病，好髮于兒童，死亡率高達40％。為進一步瞭解兒童HLH的髮病機製，研究利用生物信息學方法篩選兒童HLH相關基因，併進行通路富集分析。方法從GEO數據庫中穫得兒童HLH 外週血單箇覈細胞基因錶達譜數據集GSE26050，利用GEO2R在線分析工具篩選差異錶達基因，隨後使用DAVID數據庫的KEGG通路富集方法對其進行分析。結果篩選齣錶達差異2倍及以上的基因184箇，其中126箇為上調基因、58箇為下調基因；富集齣3條通路：細胞因子-細胞因子受體相互作用、造血細胞譜及NOD樣受體通路。結論通過生物信息學方法可篩選齣兒童HLH相關基因及與之髮生髮展密切相關的生物通路，為進一步揭示兒童HLH髮病機製提供實驗參攷。
목적：서혈세포성림파조직세포증생증（ hemophagocytic lymphohistiocytosis ， HLH）시일충치명적과도염증성질병，호발우인동，사망솔고체40％。위진일보료해인동HLH적발병궤제，연구이용생물신식학방법사선인동HLH상관기인，병진행통로부집분석。방법종GEO수거고중획득인동HLH 외주혈단개핵세포기인표체보수거집GSE26050，이용GEO2R재선분석공구사선차이표체기인，수후사용DAVID수거고적KEGG통로부집방법대기진행분석。결과사선출표체차이2배급이상적기인184개，기중126개위상조기인、58개위하조기인；부집출3조통로：세포인자-세포인자수체상호작용、조혈세포보급NOD양수체통로。결론통과생물신식학방법가사선출인동HLH상관기인급여지발생발전밀절상관적생물통로，위진일보게시인동HLH발병궤제제공실험삼고。
Objective Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by excessive inflammation, with a high incidence in children and a death rate of 40%.This study was to analyze the gene expression profile in child-hood HLH and explore the important pathways of childhood HLH using bioinformatic methods . Methods The childhood HLH gene ex-pression profile data GSE26050 were obtained from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnolo-gy Information.Differentially expressed genes were identified with the GEO 2R online analysis tools released recently .The key pathways of the differentially expressed genes were investigated using the Kyoto Encyclopedia of Genes and Genomes ( KEGG) pathway enrichment a-nalysis. Results A total of 184 differentially expressed genes were identified , 126 upregulated and the other 58 downregulated .They were enriched in 3 pathways, including cytokine-cytokine receptor interaction , hematopoietic cell lineage and NOD-like receptor signaling pathways. Conclusion Bioinformatic tools allow the identification of the key genes and pathways associated with the development and progression of childhood HLH and point out the potential directions for researches on the mechanisms of childhood HLH .
