南昌大学学报（医学版）
南昌大學學報（醫學版）
남창대학학보（의학판）
ACTA ACADEMIAE MEDICINAE JIANGXI
2014年
3期
6-9
,共4页
郭萍%陈贺%张晓玲%胡川%匡渤海
郭萍%陳賀%張曉玲%鬍川%劻渤海
곽평%진하%장효령%호천%광발해
子宫内膜异位症%S100A4 基因%表达
子宮內膜異位癥%S100A4 基因%錶達
자궁내막이위증%S100A4 기인%표체
endometriosis%S100A4%expression
目的：探讨 S100A4基因在子宫内膜异位症（endometriosis，EMs）及正常人在位内膜细胞中的表达及意义。方法通过细胞培养、细胞鉴定及定量逆转录聚合酶链反应（QRT-PCR）方法检测30例 EMs 在位内膜细胞（EMs组）及30例正常子宫在位内膜细胞（对照组）S100A4基因的表达情况。结果Quantity One 等定量软件分析结果发现 EMs 组 S100A4蛋白灰度值明显高于对照组（P ＜0．05）；通过 QRT-PCR 系统软件分析 EMs 组患者的S100A4 mRNA 表达量比对照组高约3倍（P ＜0．001）。结论 EMs 在位内膜细胞中 S100A4基因表达增加， S100A4基因差异表达与 EMs 侵袭和转移有关。
目的：探討 S100A4基因在子宮內膜異位癥（endometriosis，EMs）及正常人在位內膜細胞中的錶達及意義。方法通過細胞培養、細胞鑒定及定量逆轉錄聚閤酶鏈反應（QRT-PCR）方法檢測30例 EMs 在位內膜細胞（EMs組）及30例正常子宮在位內膜細胞（對照組）S100A4基因的錶達情況。結果Quantity One 等定量軟件分析結果髮現 EMs 組 S100A4蛋白灰度值明顯高于對照組（P ＜0．05）；通過 QRT-PCR 繫統軟件分析 EMs 組患者的S100A4 mRNA 錶達量比對照組高約3倍（P ＜0．001）。結論 EMs 在位內膜細胞中 S100A4基因錶達增加， S100A4基因差異錶達與 EMs 侵襲和轉移有關。
목적：탐토 S100A4기인재자궁내막이위증（endometriosis，EMs）급정상인재위내막세포중적표체급의의。방법통과세포배양、세포감정급정량역전록취합매련반응（QRT-PCR）방법검측30례 EMs 재위내막세포（EMs조）급30례정상자궁재위내막세포（대조조）S100A4기인적표체정황。결과Quantity One 등정량연건분석결과발현 EMs 조 S100A4단백회도치명현고우대조조（P ＜0．05）；통과 QRT-PCR 계통연건분석 EMs 조환자적S100A4 mRNA 표체량비대조조고약3배（P ＜0．001）。결론 EMs 재위내막세포중 S100A4기인표체증가， S100A4기인차이표체여 EMs 침습화전이유관。
Objective To investigate the expression and significance of S100A4 gene in endom-etrial cells of endometriosis(EMs)patients and normal subjects.Methods Endometrial cells from 30 EMs patients(EMs group)and 30 normal subjects(control group)were cultured and identi-fied.The expression of S100A4 was detected by Western-blot and QRT-PCR.Results The Quan-tity One analysis showed that the grey value of S100A4 protein in EMs group was significantly greater than that in control group (P < 0.05).The QRT-PCR showed that the expression of S100A4 mRNA in EMs group was 3 times higher than that in control group(P <0.001).Conclu-sion The expression of S100A4 increases in EMs and the increased expression is correlated with the invasion and metastasis of EMs.
