CAJ | 학술논문

目的：高迁移率族蛋白B1（high mobility group box 1， HMGB1）参与炎症免疫反应及细胞的增殖、分化、迁移等，但肝癌组织HMGB1的表达情况研究甚少。文中旨在观察HMGB1在肝细胞癌（ hepatocellular carcinoma ， HCC）组织中基因和蛋白表达水平的改变。方法使用实时荧光定量PCR和免疫印迹法分别检测13例HCC组织及其癌旁组织中HMGB1 mRNA和蛋白表达水平，组织免疫荧光定位分析肝组织细胞内HMGB1分布的变化。结果 HCC组织中HMGB1 mRNA和蛋白表达水平[（4．01±0．81）和（1．37±0．34）]均显著高于癌旁组织[（5．76±1．28）和（0．52±0．12）]，差异有统计学意义（P＜0．01），肝癌细胞显示HMGB1核浆转位。结论 HCC组织HMGB1表达增强，HMGB1参与肝细胞癌的发生发展过程。
목적：고천이솔족단백B1（high mobility group box 1， HMGB1）삼여염증면역반응급세포적증식、분화、천이등，단간암조직HMGB1적표체정황연구심소。문중지재관찰HMGB1재간세포암（ hepatocellular carcinoma ， HCC）조직중기인화단백표체수평적개변。방법사용실시형광정량PCR화면역인적법분별검측13례HCC조직급기암방조직중HMGB1 mRNA화단백표체수평，조직면역형광정위분석간조직세포내HMGB1분포적변화。결과 HCC조직중HMGB1 mRNA화단백표체수평[（4．01±0．81）화（1．37±0．34）]균현저고우암방조직[（5．76±1．28）화（0．52±0．12）]，차이유통계학의의（P＜0．01），간암세포현시HMGB1핵장전위。결론 HCC조직HMGB1표체증강，HMGB1삼여간세포암적발생발전과정。
Objective High mobility group box 1 (HMGB1) mediates the inflammatory immune response as well as the prolif-eration, differentiation and migration of cells , neverthless, little study has been done on the changes of HMGB 1 expression in hepatoma tissues.The aim of the article was to investigate the changes of HMGB 1 mRNA and protein expression in hepatocellular carcinoma (HCC) tissues. Methods Real-time fluorescence quantitative PCR and Western blotting were applied to detect HMGB 1 mRNA and protein expression in HCC tissues and their paracancerous tissues of 13 cases.Immunofluorescence localization was used to analyze the changes of HMGB1 distribution in hepatic cells . Results The expression of HMGB1 mRNA and protein in HCC tissues (4.01 ±0.81) and (1.37 ±0.34) increased significantly in comparison with those in paracancerous tissues (5.76 ±1.28) and (0.52 ±0.12) ( P<0.01).Nucleo-cytoplasmic translocation of HMGB1 was observed in hepatocarcinoma cells . Conclusion There is increased expression of HMGB1 in HCC tissues, which shows HMGB1 mediates the carcinogenesis of HCC .