CAJ | 학술논문

目的：探讨宫颈癌组织中miR-9表达，并对靶基因进行预测及生物信息学分析，为深入研究miR-9的调控机制及生物学功能提供理论依据。方法应用miRNAs芯片检测宫颈癌组织及正常宫颈组织中miRNAs表达，利用生物学软件对其中上调较明显的miR-9进行靶基因预测，同时利用基因芯片技术筛选转染miR-9后宫颈癌Hela细胞中差异表达的基因，将两者预测靶基因的交集作为miR-9的靶基因进行GO富集分析和生物通路富集分析。结果与正常宫颈组织比较，宫颈癌组织中有15个miRNAs表达为上调，10个miRNAs表达为下调，其中上调较明显的是miR-21和miR-9，下调较明显的是miR-376a和miR-218。芯片所示的miR-9调控的下调基因与软件预测的靶基因交集中共有148个基因，miR-9的预测靶基因富集在细胞内的生物学调控、合成和代谢等生物学过程以及转录调控和蛋白结合等分子功能上，其信号通路富集于调节肌动蛋白细胞骨架、脂质代谢和细胞黏附通路。结论 miR-9在宫颈癌组织中高表达，miR-9预测的靶基因富集于多个生物学功能和生物学过程及与肿瘤相关的信号通路。
목적：탐토궁경암조직중miR-9표체，병대파기인진행예측급생물신식학분석，위심입연구miR-9적조공궤제급생물학공능제공이론의거。방법응용miRNAs심편검측궁경암조직급정상궁경조직중miRNAs표체，이용생물학연건대기중상조교명현적miR-9진행파기인예측，동시이용기인심편기술사선전염miR-9후궁경암Hela세포중차이표체적기인，장량자예측파기인적교집작위miR-9적파기인진행GO부집분석화생물통로부집분석。결과여정상궁경조직비교，궁경암조직중유15개miRNAs표체위상조，10개miRNAs표체위하조，기중상조교명현적시miR-21화miR-9，하조교명현적시miR-376a화miR-218。심편소시적miR-9조공적하조기인여연건예측적파기인교집중공유148개기인，miR-9적예측파기인부집재세포내적생물학조공、합성화대사등생물학과정이급전록조공화단백결합등분자공능상，기신호통로부집우조절기동단백세포골가、지질대사화세포점부통로。결론 miR-9재궁경암조직중고표체，miR-9예측적파기인부집우다개생물학공능화생물학과정급여종류상관적신호통로。
Objective To investigate the expression of microRNA ( miRNA) in cervical cancer tissues and its predicted target genes to provide a theoretical basis for the study on the regulatory mechanism and biological functions of miR-9 .Methods MiRNA microarray was applied to detecting the miRNAs expressions in cervical caner tissues and normal cercvical tissues .The biological software was used to predict target genes of up-regulate miRNA-9.The miR-9 mimics were transected into cervical cancer cell line Hela ,and the mRNA microarrays was used to examine the expression of downstream genes of miR-9.The bioinformatics analysis of the target genes of miR-9 involved gene ontology and signal transduction pathway enrichment .Results Compared with normal cervical tissues ,there were 15 miRNAs up-expression and 10 miRNAs down-expression in cervical cancer tissues .miR-21 and miR-9 was significantly up-regulated while miR-376a and miR-218 were significantly down-regulated.The mRNA microarrays showed that 148 genes were down expression in miR-9,which also existed in network database ,and the genes set mainly located in biosynthetic and metabolic process as well as transcription and protein binding function .The KEGG pathway analysis demonstrated that the gene set mostly located in the regulation of actin cytoskeleton,sphingolipid metabolism and focal adhesion.Conclusion miR-9 expression significantly increases in cervical cancer tissues .The target genes set of miR-9 enriches in multiple biological function and process as well as tumor-related signaling pathways .