CAJ | 학술논문

目的：观察针灸针斜刺阿是穴对家兔腓肠肌急性钝挫伤修复的形态学影响。方法：采用定量负荷自由落体复制肌肉急性闭合性钝挫伤的方法对模型家兔右后肢腓肠肌进行造模，分别于造模后即刻、造模后24 h、造模后48 h测量腓肠肌肿胀度，肌肉硬度，于造模后48 h取材，并检测血清中肌酸激酶、乳酸脱氢酶含量，并通过HE染色光镜和透射电镜观察家兔腓肠肌形态学改变。结果：造模后48 h各组家兔血清肌酸激酶（CK）含量比较，与正常组相比，模型组、针刺组、CsA模型组、CsA针刺组均显著身高（P＜0.05），与模型组相比，CsA模型组显著升高（P＜0.05）；造模后48 h各组家兔乳酸脱氢酶（LDH）含量，与空白组比较，模型组和CsA模型组显著升高（P＜0.05），与模型组相比，针刺组和CsA针刺组显著降低（P＜0.05）；HE染色光镜下，模型组及各干预组可见不同程度的肌纤维损害；透射电镜下模型组及各干预组可见不同程度的超微结构变化。结论：本实验中的造模方法可以成功复制腓肠肌急性闭合性钝挫伤模型，针灸针斜刺阿是穴可有效地促进腓肠肌急性钝挫伤修复，环孢素干预一定程度降低了针刺的修复作用。
목적：관찰침구침사자아시혈대가토비장기급성둔좌상수복적형태학영향。방법：채용정량부하자유락체복제기육급성폐합성둔좌상적방법대모형가토우후지비장기진행조모，분별우조모후즉각、조모후24 h、조모후48 h측량비장기종창도，기육경도，우조모후48 h취재，병검측혈청중기산격매、유산탈경매함량，병통과HE염색광경화투사전경관찰가토비장기형태학개변。결과：조모후48 h각조가토혈청기산격매（CK）함량비교，여정상조상비，모형조、침자조、CsA모형조、CsA침자조균현저신고（P＜0.05），여모형조상비，CsA모형조현저승고（P＜0.05）；조모후48 h각조가토유산탈경매（LDH）함량，여공백조비교，모형조화CsA모형조현저승고（P＜0.05），여모형조상비，침자조화CsA침자조현저강저（P＜0.05）；HE염색광경하，모형조급각간예조가견불동정도적기섬유손해；투사전경하모형조급각간예조가견불동정도적초미결구변화。결론：본실험중적조모방법가이성공복제비장기급성폐합성둔좌상모형，침구침사자아시혈가유효지촉진비장기급성둔좌상수복，배포소간예일정정도강저료침자적수복작용。
Objective:To observe the morphological effects of acupuncture on Ashi points of rabbits with acute gastrocnemius blunt con -tusion.Methods:Modeling was given on gastrocnemius muscle in rabits'hindquarter by using method of free falling with quantitative load to duplicate acute skeletal muscle injury .The swelling degree and hardness of gastrocnemius ( immediately, 24hours, 48hours after building the model) was measured.Materials were taken 48hours after building the model.The content of CK and LDH in serum were tested.The morphological changes of gastrocnemius under light microscope and electron microscope were observed .Results:48 hours after building the model , the content of CK of model group , acupuncture group , CsA model group and CsA acupuncture group increased significantly compared the nature group (P<0.05);compared with the nature group, differences of the content of LDH of model group and CsA model group increased significantly ( P<0.05 ); compared with the model group , acupuncture group , CsA model group and CsA acupuncture group decreased significantly ( P<0.05 ) .Under light microscope , different kinds of muscle fiber trauma could be found in all groups except the nature group .Under electron microscope , Ultrastructural changes could be seen in all groups except the nature group .Conclusion:The modeling method can successfully copy acute closed skeletal gastrocnemius muscle injury model .Acu-puncture could promote the reparative process of acute blunt muscle effectively , while CsA would partially reduce the repairing effect of acupuncture .