CAJ | 학술논문

目的：探讨蜈蚣三七纳米粉预防大鼠术后肠粘连的作用机制。方法：Ellis法建立大鼠术后肠粘连模型，随机分为假手术组、模型组、阳性组（肌内注射地塞米松10 mg/kg）、蜈蚣三七高、中、低剂量组（分别灌胃450、225、112 mg/kg）。术前3 d开始给药，术后继续给药1周。末次给药后，取血清，ELISA法检测TNF-α、IL-1β和IL-6的表达，并进行粘连程度肉眼分级。结果：与模型组比较，蜈蚣三七纳米粉各剂量均能明显改善肠粘连程度（ P＜0.05或P＜0.01），均可明显降低血清TNF-α、IL-1β和IL-6水平（P＜0.05或P＜0.01）。结论：蜈蚣三七纳米粉可抑制炎性反应因子的过度表达，减轻炎性反应，有效预防术后肠粘连的发生。
목적：탐토오공삼칠납미분예방대서술후장점련적작용궤제。방법：Ellis법건립대서술후장점련모형，수궤분위가수술조、모형조、양성조（기내주사지새미송10 mg/kg）、오공삼칠고、중、저제량조（분별관위450、225、112 mg/kg）。술전3 d개시급약，술후계속급약1주。말차급약후，취혈청，ELISA법검측TNF-α、IL-1β화IL-6적표체，병진행점련정도육안분급。결과：여모형조비교，오공삼칠납미분각제량균능명현개선장점련정도（ P＜0.05혹P＜0.01），균가명현강저혈청TNF-α、IL-1β화IL-6수평（P＜0.05혹P＜0.01）。결론：오공삼칠납미분가억제염성반응인자적과도표체，감경염성반응，유효예방술후장점련적발생。
Objective:To investigate the anti-inflammatory mechanism of nano-powder of Wugongsanqi ( NW) , the rhizome of Anemone flaccid, on postoperative intestinal adhesion in rats .Methods:Fifty SD rats were subjected to operation with Ellis'method for establis-hing intestinal adhesion models , then randomly divided into 5 groups (n=10), namely model, positive (Dexamethasone, i.m.10 mg/kg), NW high, medium and low dose group (p.o.450, 225 and 112 mg/kg, respectively).Another ten normal rats were selected as control group .Drugs were given 3 days before the operation until 7 days after the operation .The intestinal adhesion was graded and the levels of TNF-α, IL-1βand IL-6 in serum were evaluated by ELISA .Results:NW evidently reduced the severity of postoperative adhe-sion (P<0.05 or P<0.01), and significantly inhibited the levels of TNF-α, IL-1βand IL-6 in serum (P<0.05 or P<0.01), com-pared with those of the model group .Conclusion:NW could effectively prevent the formation of postoperative intestinal adhesion by in -hibiting the expression of inflammatory cytokines and improving the inflammatory response .