CAJ | 학술논문

目的：观察补肾通督胶囊对肾虚寒凝型类风湿性关节炎患者外周血Th17/Treg细胞及血清白细胞介素17A（ IL-17A）、转化生长因子-β1（TGF-β1）、IL-10的影响。方法：入选类风湿性关节炎患者随机双盲分为治疗组（36例）和对照组（35例），治疗组口服补肾通督胶囊，对照组口服雷公藤多苷片，均服药12周，采用流式细胞术观察比较治疗前后两组患者外周血Th17/Treg及采用酶联免疫吸附法（ELISA）观察血清IL-17A、TGF-β1、IL-10的变化。结果：治疗后两组与各自治疗前相比， Th17及Th17/Treg显著降低（P＜0.01），Treg显著升高（P＜0.01）；治疗后两组间比较无统计学意义（P＞0.05）。治疗后两组与各自组治疗前相比，IL-17A显著降低（P＜0.01），TGF-β1、IL-10显著升高（P＜0.01）；治疗后两组间比较，治疗组TGF-β1与IL-10升高更加明显（P＜0.01），IL-17A无统计学意义（P＞0.05）。结论：补肾通督胶囊可以明显降低肾虚寒凝型类风湿性关节炎患者外周血Th17/Treg水平，降低血清IL-17A含量，而使血清TGF-β1、IL-10含量升高。
목적：관찰보신통독효낭대신허한응형류풍습성관절염환자외주혈Th17/Treg세포급혈청백세포개소17A（ IL-17A）、전화생장인자-β1（TGF-β1）、IL-10적영향。방법：입선류풍습성관절염환자수궤쌍맹분위치료조（36례）화대조조（35례），치료조구복보신통독효낭，대조조구복뢰공등다감편，균복약12주，채용류식세포술관찰비교치료전후량조환자외주혈Th17/Treg급채용매련면역흡부법（ELISA）관찰혈청IL-17A、TGF-β1、IL-10적변화。결과：치료후량조여각자치료전상비， Th17급Th17/Treg현저강저（P＜0.01），Treg현저승고（P＜0.01）；치료후량조간비교무통계학의의（P＞0.05）。치료후량조여각자조치료전상비，IL-17A현저강저（P＜0.01），TGF-β1、IL-10현저승고（P＜0.01）；치료후량조간비교，치료조TGF-β1여IL-10승고경가명현（P＜0.01），IL-17A무통계학의의（P＞0.05）。결론：보신통독효낭가이명현강저신허한응형류풍습성관절염환자외주혈Th17/Treg수평，강저혈청IL-17A함량，이사혈청TGF-β1、IL-10함량승고。
Objective:To investigate the effect of Bushen Tongdu capsule on peripheral Th 17/Treg ratio and serum IL-17A,TGF-β1 and IL-10 of patients with rheumatoid arthritis ( RA) .Methods:RA patients were divided randomly and double-blinded into treatment group ( n=36) that were treated with Bushen Tongdu capsules and control group ( n=35) that were treated with Tripterygium glycosides tab-lets.Patients in both groups were treated for 12 weeks.The changes of peripheral blood Th 17/Treg ratio and serum IL-17A,TGF-β1 and IL-10 were observed by flow cytometry and ELISA in patients of both teams before and after the treatments .Results:After treatments , Pe-ripheral Th 17%,Th17/Treg ratio significantly decreased and Treg%significantly evaluated ( P<0.01 ) in both groups , and there was no statistically significant difference between the two groups after the treatments (P>0.05).After treatments, the level of serum IL-17A significantly decreased, and TGF-β1 and IL-10 significantly evaluated in both groups (P<0.01).Comparing the two groups, TGF-β1 and IL-10 evaluated more in treatment group (P<0.01), while there was no statistically significant difference between the two groups on the changes of IL-17A(P>0.05).Conclusion:Bushen Tongdu capsule can reduce RA patients`peripheral Th17/Treg ratio and ser-um IL-17A content, and it also can evaluate serum TGF-β1 and IL-10 content.