CAJ | 학술논문

目的：观察溃疡性结肠炎（ Ulcerative Colitis ，UC）大鼠在“肠病及肺”过程中呼吸道和肠道微生态的同步动态变化情况，探讨中医“肺与大肠相表里”理论的微生态机制。方法：以三硝基苯磺酸（ TNBS）-乙醇相结合的方法诱导实验性大鼠UC模型，分别在造模后第8天、第29天和第50天三个时间点对呼吸道和肠道的需氧菌总数、厌氧菌总数、肠杆菌、肠球菌、葡萄球菌、产气荚膜梭菌、双歧杆菌、乳酸杆菌进行同步检测。结果：UC大鼠出现肠道菌群失调，益生菌数量减少，条件致病菌数量增多。其呼吸道部分菌群同步出现相关变化。造模后第8天，需氧菌总数和葡萄球菌在呼吸道和肠道同步增多，厌氧菌总数和肠杆菌在肠道增多而在呼吸道减少。造模后第29天，需氧菌总数和葡萄球菌在呼吸道和肠道同步减少，厌氧菌总数和肠杆菌在肠道减少而在呼吸道增多；造模后第50天，呼吸道和肠道的需氧菌总数、厌氧菌总数和葡萄球菌在呼吸道和肠道同步增多。结论：肠病大鼠可出现呼吸道菌群的改变，在“肠病及肺”病理传变过程中，肠病大鼠呼吸道和肠道的部分菌群出现同步增多或减少的相关性变化，提示微生态菌群的变化可能是“肠病及肺”的机制和表现形式之一。
목적：관찰궤양성결장염（ Ulcerative Colitis ，UC）대서재“장병급폐”과정중호흡도화장도미생태적동보동태변화정황，탐토중의“폐여대장상표리”이론적미생태궤제。방법：이삼초기분광산（ TNBS）-을순상결합적방법유도실험성대서UC모형，분별재조모후제8천、제29천화제50천삼개시간점대호흡도화장도적수양균총수、염양균총수、장간균、장구균、포도구균、산기협막사균、쌍기간균、유산간균진행동보검측。결과：UC대서출현장도균군실조，익생균수량감소，조건치병균수량증다。기호흡도부분균군동보출현상관변화。조모후제8천，수양균총수화포도구균재호흡도화장도동보증다，염양균총수화장간균재장도증다이재호흡도감소。조모후제29천，수양균총수화포도구균재호흡도화장도동보감소，염양균총수화장간균재장도감소이재호흡도증다；조모후제50천，호흡도화장도적수양균총수、염양균총수화포도구균재호흡도화장도동보증다。결론：장병대서가출현호흡도균군적개변，재“장병급폐”병리전변과정중，장병대서호흡도화장도적부분균군출현동보증다혹감소적상관성변화，제시미생태균군적변화가능시“장병급폐”적궤제화표현형식지일。
Objective:To explore the micro ecological mechanism of the TCM theory “lung and large intestine are exteriorly and interi-orly related”via observing the synchronous dynamic change of microecology of respiratory and intestinal passage of rats with ulcerative co -litis.Methods:The ulcerative colitis rat models were created by TNBS (trinitro-benzene-sulfonic acid).We synchronously detected to-tal aerobic bacteria , anaerobic bacteria , enterobacterium , enterococcus , staphylococcus , clostridium perfringens , bifidobacterium and lactobacillus in respiratory and intestinal passage on the 8th day, the 29th day and the 50th day after molding.Results:Dysbacteriosis appeared among the rat models including probiotics decreased and conditional pathogenic bacteria increased .Meanwhile , part of the re-spiratory floras of the model rats also appeared certain relative changes .Aerobe total and staphylococcus synchronously increased both in lung and large intestine on the 8th day while anaerobe total and enterobacterium increased in intestine but decreased in lung .Aerobe to-tal and staphylococcus synchronously decreased both in lung and large intestine at the 29th day while anaerobe total and enterobacterium decreased in intestine but increased in lung .Aerobe total, anaerobe total and staphylococcus synchronously increased both in lung and large intestine at the 50th day.Conclusion:Rats with enteropathy also can suffer from changes of respiratory floras .Parts of the respira-tory and intestinal floras of rats with enteropathy appear synchronous changes during the process of enteropthy affected lung .It may in-crease or decreased synchronously .Changes in microbial floras can be one of the mechanisms and forms of “enteropathy affected lung”.