CAJ | 학술논문

目的：研究清胰化积方干预后 MMPs 的表达变化对胰腺癌肝转移的影响。方法：通过脾脏注射法建立裸鼠人胰腺癌SW1990HM肝转移模型，观察清胰化积方干预后对胰腺癌肝转移灶增殖的影响，通过检测MMPs mRNA表达的变化观察其与肝转移灶之间的关系，并通过免疫组化和western blot进一步验证。结果：①与NS组相比，QYHJ各组均可抑制肝转移灶的增殖生长（P<0.05）；②与NS组比较，QYHJ干预后，大、中、小剂量组的肝转移灶肿瘤组织中MMP-1、MMP-7、MMP-9的mRNA表达无明显差异（VS NS，P>0.05）；MMP-2的mRNA表达下调（VS NS，P<0.05）。结论：清胰化积方可能通过诱导MMP-2的表达下调抑制胰腺癌肝转移的发展。
목적：연구청이화적방간예후 MMPs 적표체변화대이선암간전이적영향。방법：통과비장주사법건립라서인이선암SW1990HM간전이모형，관찰청이화적방간예후대이선암간전이조증식적영향，통과검측MMPs mRNA표체적변화관찰기여간전이조지간적관계，병통과면역조화화western blot진일보험증。결과：①여NS조상비，QYHJ각조균가억제간전이조적증식생장（P<0.05）；②여NS조비교，QYHJ간예후，대、중、소제량조적간전이조종류조직중MMP-1、MMP-7、MMP-9적mRNA표체무명현차이（VS NS，P>0.05）；MMP-2적mRNA표체하조（VS NS，P<0.05）。결론：청이화적방가능통과유도MMP-2적표체하조억제이선암간전이적발전。
Objective:To study the effects of Qingyi on expression of MMPs in pancreatic cancer for liver metastasis. Methods:To building human pancreatic cancer SW1990HM liver metastasis model in nude mice by injection of spleen, observing effects of the Qinyi Huaji recipe in liver metastases proliferation from pancreatic cancer, by detecting changes in the expression of MMPs mRNA and verified by immunohistochemistry and western blot. Results:I. Compared with NS group, QYHJ group can inhibit the proliferation of the growth of liver metastases (P<0.05); II. Compared with NS, with QYHJ intervention, in large liver metastases tumor tissue, small-dose group, MMP-1, MMP-7, mRNA MMP-9 expression were no significant difference (VS NS, P>0.05);mRNA MMP-2 is downregulated (VS NS, P <0.05). Conclusion:QYHJ party can induce the expression of MMP-2 downregulation to inhibit the development of pancreatic cancer liver metastasis.