化工进展
化工進展
화공진전
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2014年
5期
1300-1305
,共6页
膜生物反应器%膜污染%生物膜%群体感应%N-乙酰高丝氨酸环内酯
膜生物反應器%膜汙染%生物膜%群體感應%N-乙酰高絲氨痠環內酯
막생물반응기%막오염%생물막%군체감응%N-을선고사안산배내지
membrane bioreactors(MBRs)%membrane fouling%biofilm%quorum sensing(QS)%N-acyl homoserine lactone(AHL)
首先介绍N-乙酰高丝氨酸环内酯（N-acyl homoserine lactone，AHL）型生物群体感应（quorum sensing， QS）信号分子对膜生物反应器（membrane bioreactor，MBR）膜表面形成生物膜的调节机制，通过AHL-QS信号分子细胞间的交流，可决定生物膜形成及胞外聚合物（extracellular polymeric substances，EPS）分泌；系统阐述了应用AHL-QS信号分子降解酶及淬灭剂对MBR生物膜污染的控制效果，抑制或降解信号分子可显著降低生物膜形成能力，从根本上控制膜污染。此外，针对降解酶及淬灭剂新的固定化技术在MBR中的应用也作了介绍，如磁性载体、膜表面负载、微生物-管束及多孔微球包埋细胞技术。以 AHL-QS 为基础的膜污染控制策略对于MBR 应用前景广阔，然而该技术的工程化研究仍有待进一步深入；加强 AHL-QS 信号分子识别及进一步明确QS系统对微生物代谢机制的影响是该领域未来重要的研究方向。
首先介紹N-乙酰高絲氨痠環內酯（N-acyl homoserine lactone，AHL）型生物群體感應（quorum sensing， QS）信號分子對膜生物反應器（membrane bioreactor，MBR）膜錶麵形成生物膜的調節機製，通過AHL-QS信號分子細胞間的交流，可決定生物膜形成及胞外聚閤物（extracellular polymeric substances，EPS）分泌；繫統闡述瞭應用AHL-QS信號分子降解酶及淬滅劑對MBR生物膜汙染的控製效果，抑製或降解信號分子可顯著降低生物膜形成能力，從根本上控製膜汙染。此外，針對降解酶及淬滅劑新的固定化技術在MBR中的應用也作瞭介紹，如磁性載體、膜錶麵負載、微生物-管束及多孔微毬包埋細胞技術。以 AHL-QS 為基礎的膜汙染控製策略對于MBR 應用前景廣闊，然而該技術的工程化研究仍有待進一步深入；加彊 AHL-QS 信號分子識彆及進一步明確QS繫統對微生物代謝機製的影響是該領域未來重要的研究方嚮。
수선개소N-을선고사안산배내지（N-acyl homoserine lactone，AHL）형생물군체감응（quorum sensing， QS）신호분자대막생물반응기（membrane bioreactor，MBR）막표면형성생물막적조절궤제，통과AHL-QS신호분자세포간적교류，가결정생물막형성급포외취합물（extracellular polymeric substances，EPS）분비；계통천술료응용AHL-QS신호분자강해매급쉬멸제대MBR생물막오염적공제효과，억제혹강해신호분자가현저강저생물막형성능력，종근본상공제막오염。차외，침대강해매급쉬멸제신적고정화기술재MBR중적응용야작료개소，여자성재체、막표면부재、미생물-관속급다공미구포매세포기술。이 AHL-QS 위기출적막오염공제책략대우MBR 응용전경엄활，연이해기술적공정화연구잉유대진일보심입；가강 AHL-QS 신호분자식별급진일보명학QS계통대미생물대사궤제적영향시해영역미래중요적연구방향。
This review described the mechanism of N-acyl homoserine lactone (AHL) style quorum sensing (QS) signal molecules for microorganisms to biofilm formation on the surface of membrane in membrane bioreactors (MBRs). Through the cell-cell communication between microorganisms,the QS determines biofilm formation and secretion of extracellular polymeric substances (EPS). The article summarized studies on the inhibition or disruption of AHL-QS signaling molecules by AHL-acylase or quorum quenching and effectively controlled membrane biofouling. The signaling molecules reduction would weaken biofilm formation ability but enhance membrane filterability. Moreover,the paper reviewed new immobilized methods about the AHL-acylase or quorum quenching,such as immobilized magnetic carriers,membrane surface,microbial-vessel and cell entrapping beads. The QS control strategies offered a promising control alternative but more investigations on the application to real MBRs were needed. Future studies should specifically address the relationship between the QS and microbial metabolism,as well as signaling molecules recognition in real MBR system.
