CAJ | 학술논문

目的观察降钙素受体(CTR)在骨肉瘤组织的表达,探讨影响骨肉瘤发展、预后的因素.方法将35例骨肉瘤标本(高分化骨肉瘤组23例和低分化骨肉瘤组12例)设为实验组,10例正常骨组织标本设为对照组,采用链霉菌抗生物素蛋白-过氧化物酶(SP)免疫组织化学方法进行染色,观察CTR的表达.结果 CTR在实验组的阳性表达率为68.6％,CTR在正常骨组织的阳性表达率为30.0％,两组间比较差异有统计学意义(P＜0.05);CTR在高分化骨肉瘤的表达率为78.2％,而在低分化骨肉瘤的表达率为33.3％,两者差异有统计学意义(P＜0.05);在高分化骨肉瘤中,CTR在男性、女性患者的阳性表达率分别为77.8％和22.2％;在低分化骨肉瘤中,CTR在男性、女性患者的阳性表达率分别为50.0％和50.0％,两组组内不同性别患者分别进行比较差异无统计学意义(P＞0.05).结论骨肉瘤上CTR表达率高于正常骨组织;CTR在高分化组比低分化组表达率高,提示其与肿瘤恶性程度和预后有关.
목적 관찰강개소수체(CTR)재골육류조직적표체,탐토영향골육류발전、예후적인소.방법 장35례골육류표본(고분화골육류조23례화저분화골육류조12례)설위실험조,10례정상골조직표본설위대조조,채용련매균항생물소단백-과양화물매(SP)면역조직화학방법진행염색,관찰CTR적표체.결과 CTR재실험조적양성표체솔위68.6％,CTR재정상골조직적양성표체솔위30.0％,량조간비교차이유통계학의의(P＜0.05);CTR재고분화골육류적표체솔위78.2％,이재저분화골육류적표체솔위33.3％,량자차이유통계학의의(P＜0.05);재고분화골육류중,CTR재남성、녀성환자적양성표체솔분별위77.8％화22.2％;재저분화골육류중,CTR재남성、녀성환자적양성표체솔분별위50.0％화50.0％,량조조내불동성별환자분별진행비교차이무통계학의의(P＞0.05).결론 골육류상CTR표체솔고우정상골조직;CTR재고분화조비저분화조표체솔고,제시기여종류악성정도화예후유관.
Objective To observe the expression of calcitonin receptor in osteosarcoma cells and explore the factors influencing the development or prognosis of osteosarcoma.Methods Thirty-five cases of osteosarcoma specimens (23 cases in well-differentiated osteosarcoma group,and 12 cases in poorly differentiated osteosarcoma group) were designed as observation group.Ten cases of normal bone tissues served as control group.All the tissues were stained by streptavid-in-peroxidase (SP) method of immunohistochemistry to analyze the expression of calcitonin receptor.Results Positive rate of calcitonin receptor in osteosarcoma cells and normal bone tissues was 68.6％ and 30.0％ respectively (P ＜ 0.05).The expression rate of calcitonin in well and poorly differentiated osteosarcoma cells was 78.2％ (female:22.2％,and male:77.8％) and 33.3％ (female:50.0％,and male:50.0％) respectively (P ＜ 0.05).There was no statistically significant difference between two genders in different groups (P ＞ 0.05).Conclusion Positive rate of calcitonin receptor in osteosarcoma cells is higher than in normal bone tissues.What's more,the expression rate of calcitonin in well-differentiated osteosarcoma cells is higher than in poorly differentiated osteosarcoma cells.Calcitonin receptor may be related to grade malignancy and prognosis of osteosarcoma.