CAJ | 학술논문

为高效制备玉米寡肽，在玉米蛋白粉 Alcalase酶水解的基础上，从 Protamex 酶、 Flavourzyme酶、Protex.7L酶、胰蛋白酶、木瓜蛋白酶等5种蛋白酶中筛选出Protex.7L酶为继续酶解玉米蛋白Alcalase酶水解物的最适用酶。通过单因素试验和响应面分析确定继续酶解制备玉米寡肽的最佳工艺参数为：加酶量1070 U · g -1、pH 8.0、温度44.0℃。在此条件下，体系的寡肽得率为（45.81±0.35）％，与理论预测值的相对误差在±1％以内，比单独应用Alcalase酶解提高25.04％。
위고효제비옥미과태，재옥미단백분 Alcalase매수해적기출상，종 Protamex 매、 Flavourzyme매、Protex.7L매、이단백매、목과단백매등5충단백매중사선출Protex.7L매위계속매해옥미단백Alcalase매수해물적최괄용매。통과단인소시험화향응면분석학정계속매해제비옥미과태적최가공예삼수위：가매량1070 U · g -1、pH 8.0、온도44.0℃。재차조건하，체계적과태득솔위（45.81±0.35）％，여이론예측치적상대오차재±1％이내，비단독응용Alcalase매해제고25.04％。
Proteases ,Protamex ,Flavourzyme ,Protex .7L ,trypsin and papain were tested for the hydrolysis of Alcalase-hydrolyzed corn gluten meal for oligo-peptide preparation . From the single factor experiment using the response surface methodology ,the optimized conditions for the enzymatic hydrolysis were found to be Protex.7 L usage at a rate of 1 070 U · g -1 ,pH at 9.0 ,and temperature at 44.0℃ .The oligo-peptide yield was up to (45.81 ± 0.35)% ,reaching the predicted maximum with a relative error of less than ± 1% .That was 25.04% higher than that when the hydrolysis was carried out using Alcalase alone .