CAJ | 학술논문

以无患子生长健壮的幼嫩枝条为材料，研究其组培快繁技术。结果表明：①芽体启动培养的最适宜培养基为MS ＋ NAA 0.2 mg · L -1＋ IBA 0.05 mg · L -1＋6-BA 3.0 mg · L -1，萌芽率最高，达94.01％；②增殖培养的最佳培养基组合为MS ＋ IBA 0.02 mg · L -1＋6-BA 1.0 mg · L -1，增殖系数最高，为4.20；③在生根培养中，1/2 MS ＋ IBA 0.5 mg · L -1＋ NAA 0.1 mg · L -1最适宜诱导生根，生根率高达80.47％。炼苗移栽后成活率高达89.21％。
이무환자생장건장적유눈지조위재료，연구기조배쾌번기술。결과표명：①아체계동배양적최괄의배양기위MS ＋ NAA 0.2 mg · L -1＋ IBA 0.05 mg · L -1＋6-BA 3.0 mg · L -1，맹아솔최고，체94.01％；②증식배양적최가배양기조합위MS ＋ IBA 0.02 mg · L -1＋6-BA 1.0 mg · L -1，증식계수최고，위4.20；③재생근배양중，1/2 MS ＋ IBA 0.5 mg · L -1＋ NAA 0.1 mg · L -1최괄의유도생근，생근솔고체80.47％。련묘이재후성활솔고체89.21％。
Vigorous young stem segment of Sapindus mukurossi were used as explants for tissue culture and fast propagation .The results showed that the optimum medium formula of bud induction culture was MS + NAA 0.2 mg · L -1 + IBA 0.05 mg · L -1 + 6-BA 3.0 mg · L -1 and the bud rate reached 94.01% .The optimum combination of multiplication culture was MS + IBA 0.02 mg · L -1 + 6-BA 1.0 mg · L -1 .During rooting culture ,1/2MS +IBA 0.5 mg · L -1 + NAA 0.1 mg · L -1 was the optimum medium .The highest rooting rate is 80.47% .After refine seeding transplant ,the survival rate was maximal for 89.21% .