浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
4期
371-375
,共5页
张静若%方芳%方剑乔%池细绿%吴丽娜
張靜若%方芳%方劍喬%池細綠%吳麗娜
장정약%방방%방검교%지세록%오려나
慢性炎性痛%电针%MrgC%NMDA受体亚基1
慢性炎性痛%電針%MrgC%NMDA受體亞基1
만성염성통%전침%MrgC%NMDA수체아기1
chronic inflammatory pain%electroacupuncture%MrgC%NR1
[目的]观察鞘内注射Mas-related G Protein-coupled C（MrgC）抗体对电针（Electroacupuncture,EA）干预完全福氏佐剂（Complete Freund's Ad-juvant,CFA）所致大鼠慢性炎性痛及脊髓背角（Spinal dorsal horn,SDH）N-甲基-D-天门冬氨酸受体亚基1（N-methyl-d-aspartate receptors1,NR1）表达的影响。[方法]SD雄性大鼠28只，脊髓鞘内插管成功后建立CFA模型，造模后随机分为生理盐水（Saline）组、MrgC抗体（MrgC Ab）组、MrgC Ab+EA组和Saline+EA组，每组7只。两EA干预组从造模24h后在患侧“足三里”和“昆仑”穴开始电针刺激，参数为2/100Hz、30min，1次/d，连续7d，其余各组均做相应固定处理。造模后7d，各组大鼠鞘内注射相应试剂。在造模前和造模后24h、6d、7d检测大鼠患足热缩退潜伏期（Thermal Paw Withdrawal Latency，T-PWL）。免疫组织化学法检测各组大鼠患侧SDH NR1的表达。[结果](1)各组大鼠在CFA造模前和造模后24h时，T-PWL均无明显差异。第6d时，与Saline组相比，MrgC Ab+EA组和Saline+EA组大鼠患足T-PWL显著延长（P<0.01）,MrgC Ab组大鼠患足T-PWL无统计学差异。第7d时，与Saline组相比，MrgC Ab组大鼠T-PWL明显缩短（P<0.05），而Saline+EA组显著延长（P<0.01）；与Saline+EA组比较，MrgC Ab+EA组大鼠T-PWL显著缩短（P<0.05）。（2）与Saline组比较，MrgC Ab组SDH NR1表达量显著提高（P<0.01）；EA干预后大鼠SDH NR1表达量显著降低（P<0.01），MrgC Ab+EA组大鼠SDHNR1表达量也显著高于Saline+EA组大鼠（P<0.05）。[结论]鞘内注射MrgC抗体能显著拮抗电针对CFA诱导性慢性炎性痛的镇痛作用，电针镇痛效应可能与下调大鼠患侧脊髓背角NR1受体表达有关。
[目的]觀察鞘內註射Mas-related G Protein-coupled C（MrgC）抗體對電針（Electroacupuncture,EA）榦預完全福氏佐劑（Complete Freund's Ad-juvant,CFA）所緻大鼠慢性炎性痛及脊髓揹角（Spinal dorsal horn,SDH）N-甲基-D-天門鼕氨痠受體亞基1（N-methyl-d-aspartate receptors1,NR1）錶達的影響。[方法]SD雄性大鼠28隻，脊髓鞘內插管成功後建立CFA模型，造模後隨機分為生理鹽水（Saline）組、MrgC抗體（MrgC Ab）組、MrgC Ab+EA組和Saline+EA組，每組7隻。兩EA榦預組從造模24h後在患側“足三裏”和“昆崙”穴開始電針刺激，參數為2/100Hz、30min，1次/d，連續7d，其餘各組均做相應固定處理。造模後7d，各組大鼠鞘內註射相應試劑。在造模前和造模後24h、6d、7d檢測大鼠患足熱縮退潛伏期（Thermal Paw Withdrawal Latency，T-PWL）。免疫組織化學法檢測各組大鼠患側SDH NR1的錶達。[結果](1)各組大鼠在CFA造模前和造模後24h時，T-PWL均無明顯差異。第6d時，與Saline組相比，MrgC Ab+EA組和Saline+EA組大鼠患足T-PWL顯著延長（P<0.01）,MrgC Ab組大鼠患足T-PWL無統計學差異。第7d時，與Saline組相比，MrgC Ab組大鼠T-PWL明顯縮短（P<0.05），而Saline+EA組顯著延長（P<0.01）；與Saline+EA組比較，MrgC Ab+EA組大鼠T-PWL顯著縮短（P<0.05）。（2）與Saline組比較，MrgC Ab組SDH NR1錶達量顯著提高（P<0.01）；EA榦預後大鼠SDH NR1錶達量顯著降低（P<0.01），MrgC Ab+EA組大鼠SDHNR1錶達量也顯著高于Saline+EA組大鼠（P<0.05）。[結論]鞘內註射MrgC抗體能顯著拮抗電針對CFA誘導性慢性炎性痛的鎮痛作用，電針鎮痛效應可能與下調大鼠患側脊髓揹角NR1受體錶達有關。
[목적]관찰초내주사Mas-related G Protein-coupled C（MrgC）항체대전침（Electroacupuncture,EA）간예완전복씨좌제（Complete Freund's Ad-juvant,CFA）소치대서만성염성통급척수배각（Spinal dorsal horn,SDH）N-갑기-D-천문동안산수체아기1（N-methyl-d-aspartate receptors1,NR1）표체적영향。[방법]SD웅성대서28지，척수초내삽관성공후건립CFA모형，조모후수궤분위생리염수（Saline）조、MrgC항체（MrgC Ab）조、MrgC Ab+EA조화Saline+EA조，매조7지。량EA간예조종조모24h후재환측“족삼리”화“곤륜”혈개시전침자격，삼수위2/100Hz、30min，1차/d，련속7d，기여각조균주상응고정처리。조모후7d，각조대서초내주사상응시제。재조모전화조모후24h、6d、7d검측대서환족열축퇴잠복기（Thermal Paw Withdrawal Latency，T-PWL）。면역조직화학법검측각조대서환측SDH NR1적표체。[결과](1)각조대서재CFA조모전화조모후24h시，T-PWL균무명현차이。제6d시，여Saline조상비，MrgC Ab+EA조화Saline+EA조대서환족T-PWL현저연장（P<0.01）,MrgC Ab조대서환족T-PWL무통계학차이。제7d시，여Saline조상비，MrgC Ab조대서T-PWL명현축단（P<0.05），이Saline+EA조현저연장（P<0.01）；여Saline+EA조비교，MrgC Ab+EA조대서T-PWL현저축단（P<0.05）。（2）여Saline조비교，MrgC Ab조SDH NR1표체량현저제고（P<0.01）；EA간예후대서SDH NR1표체량현저강저（P<0.01），MrgC Ab+EA조대서SDHNR1표체량야현저고우Saline+EA조대서（P<0.05）。[결론]초내주사MrgC항체능현저길항전침대CFA유도성만성염성통적진통작용，전침진통효응가능여하조대서환측척수배각NR1수체표체유관。
[Objective] To observe whether the intrathecal injection has an impact on the effect of electroacupuncture(EA) intervening on chronic inflammatory pain of rats that caused by complete Freund's adjuvant(CFA) and on the expression of spinal dorsal horn N-methyl-d-aspartate sub unit1 (NR1). [Methods] SD rats 28, each right hindfoot of rats was modeled with CFA after the success of the spinal cord sheath intubation.Al rats were randomly divided into four groups:saline,MrgC antibody ,MrgC antibody+EA and saline+EA. Each group of seven.Detect the thermol paw withdrawal latency(T-PWL) of rats 24h later,then startto intervene on the groups that EA is included at two points of affected side("Zusanli" and "kunlun"), 2/100 Hz, 30 min, once a day and continuously for 7 days. Meanwhile, the other groups treated the same except for EA. On the 7d,each group was intrathecal injected of corresponding reagent.We tested the T-PWL of rats before the CFA modeled, 24h after modeled, the 6d and the 7d. The expression of NR1 in the spinal dorsal horn of each rats was detected by the method of immune histochemical. [Results] (1)The T-PWL of rats detected before CFA modeling and after modeling for 24h showed that each group was not statistical y different.The 6d, compared with Saline group, the T-PWL of MrgCAb +EA group and Saline + EA group rats were significantly prolonged( P<0.01,P<0.01), while the MrgCAb group was not statistical y different. The 7d, compared with Saline, the T-PWL of MrgC Ab group was significantly shortened but Saline+EA group was significantly prolonged( P<0.05, P<0.01);The T-PWL of MrgC Ab+EA group rats were no shorter than the Saline group, but significantly shorter than Saline+EA group .(2)Compared with Saline group, the expression of SDH NR1 was significantly increased in MrgC Ab group while decreased after intervened by EA( P<0.01). Compared with Saline+EA group, the expression of SDH NR1 was significantly improved in the MrgCAb+EAgroup. [Conclusion]The analgesic effect of electroacu-puncture on chronicinflammatory pain induced by CFA would be strongly inhibited by intrathecal injectionof MrgC antibody,which may play a role in downregulating the expression of NR1 receptor distributed in the affected side spinal dorsal horn of rats.
