CAJ | 학술논문

目的：观察脑梗死大鼠经人脐带间充质干细胞（umbilical cord-derived mesenchymal stem cells，UC-MSCs）治疗后，行为学的恢复情况，2，3，5-氯化三苯基四氮唑（triphenyl tetrazolium chloride，TTC）染色以及胆碱能的变化。方法制备脑梗死大鼠大脑中动脉闭塞（Middle cerebral artery occlusion，MCAO）模型，将 UC-MSCs 注入脑梗死大鼠体内，对大鼠运动功能进行评分，比较 TTC 染色显示的脑梗死区域所占比例，测定脑组织和血浆中乙酰胆碱酯酶（True choline esterase，TChE）活性和乙酰胆碱（Acetylcholine，Ach）浓度。结果与模型组相比，UC-MSCs 组大鼠行为学恢复明显（P ＜0．05），UC-MSCs 组大鼠脑组织和血浆乙酰胆碱酯酶（TChE）和乙酰胆碱（Ach）活性显著增强（P＜0．05）。结论UC-MSCs 经尾静脉移植可促进脑梗死后神经功能恢复，增强大鼠胆碱能系统活性。
목적：관찰뇌경사대서경인제대간충질간세포（umbilical cord-derived mesenchymal stem cells，UC-MSCs）치료후，행위학적회복정황，2，3，5-록화삼분기사담서（triphenyl tetrazolium chloride，TTC）염색이급담감능적변화。방법제비뇌경사대서대뇌중동맥폐새（Middle cerebral artery occlusion，MCAO）모형，장 UC-MSCs 주입뇌경사대서체내，대대서운동공능진행평분，비교 TTC 염색현시적뇌경사구역소점비례，측정뇌조직화혈장중을선담감지매（True choline esterase，TChE）활성화을선담감（Acetylcholine，Ach）농도。결과여모형조상비，UC-MSCs 조대서행위학회복명현（P ＜0．05），UC-MSCs 조대서뇌조직화혈장을선담감지매（TChE）화을선담감（Ach）활성현저증강（P＜0．05）。결론UC-MSCs 경미정맥이식가촉진뇌경사후신경공능회복，증강대서담감능계통활성。
Objective To observe the effects of intravenous administration of human umbilical cord-derived mesen-chymal stem cells(UC-MSCs)after middle cerebral artery occlusion(MCAO)in adult rats.Methods Adult male SD rats were administrated by MCAO.Functional outcome measurements using Beam walking Test(BWT)were performed at 24 hour of MCAO group and 10 days of UC-MSCs treatment group respectively.TTC staining ,TChE and Ach activity array were also used.Results There was significant neurological function improvement in rats treated with MSC com-paring with that of control groups(P <0.05),and also Ach and TChE activity (P <0.05).Conclusion Intravenous ad-ministration of UC-MSCs promotes the neurological function in rat MCAO model.UC-MSCs play an advantageous function on cholinergic neurotransmitters in the case of lesion.