CAJ | 학술논문

目的：观察植入式皮层电刺激对缺血性脑卒中模型大鼠微管相关蛋白-2表达的影响。方法选择105只健康成年 SPF 级雄性 SD 大鼠，采用线栓法制作大脑中动脉阻塞模型后，MRI 筛选24只 Bederson 评分为1-4分的皮层梗死大鼠。随机分为电刺激组13只和非刺激组11只。造模1周后，两组大鼠均植入电刺激器，电极放置在脑梗死区边缘皮层表面。非刺激组大鼠植入刺激器后不施加电刺激，电刺激组大鼠给予电刺激，电刺激组大鼠在植入电刺激器2周后终止电刺激。6周后，采用免疫荧光染色法和免疫荧光图像分析系统定量分析比较两组大鼠梗死灶周边及梗死对侧皮层微管相关蛋白-2的表达水平。结果电刺激组大鼠梗死灶周边及梗死对侧皮层微管相关蛋白-2的表达水平均高于非刺激组（t＝12．189、2．454，P ＜0．05）。结论单独植入式皮层电刺激治疗缺血性脑卒中大鼠，能够增加梗死灶周围及梗死灶对侧皮层微管相关蛋白-2的表达水平，诱导双侧皮层神经元的可塑性改变。
목적：관찰식입식피층전자격대결혈성뇌졸중모형대서미관상관단백-2표체적영향。방법선택105지건강성년 SPF 급웅성 SD 대서，채용선전법제작대뇌중동맥조새모형후，MRI 사선24지 Bederson 평분위1-4분적피층경사대서。수궤분위전자격조13지화비자격조11지。조모1주후，량조대서균식입전자격기，전겁방치재뇌경사구변연피층표면。비자격조대서식입자격기후불시가전자격，전자격조대서급여전자격，전자격조대서재식입전자격기2주후종지전자격。6주후，채용면역형광염색법화면역형광도상분석계통정량분석비교량조대서경사조주변급경사대측피층미관상관단백-2적표체수평。결과전자격조대서경사조주변급경사대측피층미관상관단백-2적표체수평균고우비자격조（t＝12．189、2．454，P ＜0．05）。결론단독식입식피층전자격치료결혈성뇌졸중대서，능구증가경사조주위급경사조대측피층미관상관단백-2적표체수평，유도쌍측피층신경원적가소성개변。
Objective To investigate the effects of cortical electrical stimulation (CES)on the expression of MAP-2 in rats model of ischemic stroke.Methods 105 healthy adult SPF male SD rats,Line bolt legal system as a model after blocked arteries in the brain,screen 24 Bederson score of 1-4 points of cortical infarction in rats through MRI.The rats with lesions in the cortex were randomly divided into CES group (n = 13)and non-stimulation group (n = 11).One week after modeled,electrical stimulators were implanted and the electrodes were placed on the surface of peri-infarct cortex.CES group was given the electric stimulation,but not in the non-stimulation group.CES was terminated after implantation for two weeks.The expression of MAP-2 in the peri-infarct and contralateral cortex was assessed by im-munofluorescence image analysis at 6 weeks after CES started.Results In the peri-infarct and contralateral cortex,the expression of MAP-2 in the CES group was higher than that in the non-stimulation group (t = 12.189、2.454,P <0.05).Conclusion Cortical electrical stimulation alone can increase the expression of MAP-2 in both peri-infarct and contralateral cortex,and induce the plasticity of bilateral cortical neuron axons.