CAJ | 학술논문

目的：探讨抑制 Smad7基因表达对 PC12细胞氧糖剥夺（OGD）损伤的影响及其机制。方法利用 PC12细胞 OGD 模型体外模拟神经细胞缺血性损伤，通过 RNA 干扰技术，沉默 Smad7基因表达，实时定量 PCR、Western blot 检测 Smad7基因的表达情况，MTT 检测下调 Smad7基因表达对细胞 OGD 损伤存活率的影响，Western blot 检测 Activin A（ActA）蛋白的表达情况。结果体外合成的 siRNA 转染可下调 Smad7基因转录及蛋白水平的表达；经OGD16 h，阳性转染组细胞存活率较阴性组显著增加，阳性转染组细胞 ActA 蛋白表达较阴性组显著升高。结论Smad7可负向调控缺血损伤诱导的 ActA/Smads 通路激活过程，抑制其表达能够发挥神经保护作用。
목적：탐토억제 Smad7기인표체대 PC12세포양당박탈（OGD）손상적영향급기궤제。방법이용 PC12세포 OGD 모형체외모의신경세포결혈성손상，통과 RNA 간우기술，침묵 Smad7기인표체，실시정량 PCR、Western blot 검측 Smad7기인적표체정황，MTT 검측하조 Smad7기인표체대세포 OGD 손상존활솔적영향，Western blot 검측 Activin A（ActA）단백적표체정황。결과체외합성적 siRNA 전염가하조 Smad7기인전록급단백수평적표체；경OGD16 h，양성전염조세포존활솔교음성조현저증가，양성전염조세포 ActA 단백표체교음성조현저승고。결론Smad7가부향조공결혈손상유도적 ActA/Smads 통로격활과정，억제기표체능구발휘신경보호작용。
Objective To explore the effect of silencing Smad7 gene in PC12 cells treated by the oxygen-glucose dep-rivation (OGD)and the related mechanism.Methods PC12 cells exposed to OGD was used as the model of neurons subjected to ischemic injury in vitro.SiRNA was applied to reduce the expression of Smad7 gene,which was detected by Real time RT-PCR and Western blot.The survival rate of cells after OGD was detected by MTT.Western blot was used to test the expression of Activin A.Results The expression of Smad7 was significantly reduced after siRNA transfec-tion.The survival rate of cells in OGD16h combined with Smad7 gene silencing group was higher than that in OGD16h group.The expression of ActA protein was increased in Smad7 gene silencing group after OGD16h treatment compared to single OGD treatment.Conclusion Smad7 gene silencing can increase the survival rate of PC12 cell to OGD injury and enhance the activation of ActA/Smads.