南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2014年
3期
171-174
,共4页
白僵菌%热雾剂%溶剂油%生物农药%孢子萌发率
白僵菌%熱霧劑%溶劑油%生物農藥%孢子萌髮率
백강균%열무제%용제유%생물농약%포자맹발솔
Beauveria bassiana%thermal aerosol%solvent oil%bio-pesticide%spore germination rate
脉冲烟雾机应用烟雾载药防治技术具有防治效率高、用药量少、环保等诸多优点,但迄今为止只用于化学农药。笔者配制了3种白僵菌制剂,检测其在25℃与50℃高温模拟存储条件下以及热力烟化后的生物活性。结果表明:在50℃高温条件下,存放1 d时的孢子萌发率下降很少,存放3 d时仍高达79?7%以上,存放7 d时最低活性可达53.1%。柴油热雾剂经过脉冲烟雾机热力烟化后的出口烟雾温度远远高于3种白僵菌制剂,且高温处理7d时的生物活性几乎全部丧失,说明柴油不能直接作为白僵菌热雾剂的溶剂油。3种白僵菌制剂经过脉冲烟雾机高温热力烟化后孢子平均萌发率达到91.3%,只比热力烟化前平均下降了0.87%。说明丙三醇与水按一定比例混合后作为生物农药热雾剂的溶剂是可行的。
脈遲煙霧機應用煙霧載藥防治技術具有防治效率高、用藥量少、環保等諸多優點,但迄今為止隻用于化學農藥。筆者配製瞭3種白僵菌製劑,檢測其在25℃與50℃高溫模擬存儲條件下以及熱力煙化後的生物活性。結果錶明:在50℃高溫條件下,存放1 d時的孢子萌髮率下降很少,存放3 d時仍高達79?7%以上,存放7 d時最低活性可達53.1%。柴油熱霧劑經過脈遲煙霧機熱力煙化後的齣口煙霧溫度遠遠高于3種白僵菌製劑,且高溫處理7d時的生物活性幾乎全部喪失,說明柴油不能直接作為白僵菌熱霧劑的溶劑油。3種白僵菌製劑經過脈遲煙霧機高溫熱力煙化後孢子平均萌髮率達到91.3%,隻比熱力煙化前平均下降瞭0.87%。說明丙三醇與水按一定比例混閤後作為生物農藥熱霧劑的溶劑是可行的。
맥충연무궤응용연무재약방치기술구유방치효솔고、용약량소、배보등제다우점,단흘금위지지용우화학농약。필자배제료3충백강균제제,검측기재25℃여50℃고온모의존저조건하이급열력연화후적생물활성。결과표명:재50℃고온조건하,존방1 d시적포자맹발솔하강흔소,존방3 d시잉고체79?7%이상,존방7 d시최저활성가체53.1%。시유열무제경과맥충연무궤열력연화후적출구연무온도원원고우3충백강균제제,차고온처리7d시적생물활성궤호전부상실,설명시유불능직접작위백강균열무제적용제유。3충백강균제제경과맥충연무궤고온열력연화후포자평균맹발솔체도91.3%,지비열력연화전평균하강료0.87%。설명병삼순여수안일정비례혼합후작위생물농약열무제적용제시가행적。
The pesticide control technology with pulse fogger has the advantages of high efficiency, less dosage, environ-mental friendly protection and others. However, it is only applied with chemical pesticides. In the paper, germination a-bility of three Beauveria bassiana preparations were tested, stored separately in temperature-controlled chamber with 25 ℃ and 50℃, and after thermal smoking. Test results on the biological activity of B. bassiana spores shown that spore germination ability decreased slightly after a day of storage, and was more than 79?7% after 3 days of storage, and reached 53?1% after 2 days of storage, in the condition of 50 ℃ high temperature simulation test. The fog temperature for diesel located on the fog ejector of pulse thermal fogger was greatly higher than the three B. bassiana preparations. So, it showed that the diesel could not be used directly as solvent oil of thermal aerosol with B. bassiana. After high tem-perature thermal atomization, the biological activity of B. bassiana preparations didn?t decrease obviously, which the fall value was only 0?87%. It was shown that mixed solution of glycerol and water in suitable proportion was feasible to apply in the solvent of thermal aerosol with bio-pesticide.