CAJ | 학술논문

利用RACE技术从杨树不定根中克隆获得PeMGP和PeJDK基因全长cDNA序列，分别包含1434 bp和1518 bp长度的开放阅读框。两者都含2个内含子，其氨基酸序列均有4个保守结构域：2个C2 H2型锌指结构域ZF1（ CX2-4 CX3 FX4 LX2 HX3-5 H）和ZF2（ CX4 CXn HXn X4 H），2个C2 HC型锌指结构域ZF3（ CX2 CXn HX3 C）和ZF4（ CXCXn HX3 C）。在根茎叶中均检测到PeMGP和PeJKD基因的表达，但它们在杨树不定根发育过程中具有动态的表达模式。杨树原生质体瞬时表达揭示，PeMGP和PeJDK蛋白都定位于细胞核，两者在细胞核发生相互作用。
이용RACE기술종양수불정근중극륭획득PeMGP화PeJDK기인전장cDNA서렬，분별포함1434 bp화1518 bp장도적개방열독광。량자도함2개내함자，기안기산서렬균유4개보수결구역：2개C2 H2형자지결구역ZF1（ CX2-4 CX3 FX4 LX2 HX3-5 H）화ZF2（ CX4 CXn HXn X4 H），2개C2 HC형자지결구역ZF3（ CX2 CXn HX3 C）화ZF4（ CXCXn HX3 C）。재근경협중균검측도PeMGP화PeJKD기인적표체，단타문재양수불정근발육과정중구유동태적표체모식。양수원생질체순시표체게시，PeMGP화PeJDK단백도정위우세포핵，량자재세포핵발생상호작용。
The PeMGP and PeJDK genes were cloned from adventitious roots of Populus, and their full-length cDNA se-quences contain open reading frame ( ORF) of 1 434 bp and 1 518 bp, respectively. There were two introns in their ge-nomic structure. The two deduced proteins all contained 4 identical zinc finger domains, ZF1 ( CX2-4 CX3 FX4 LX2 HX3-5 H) and ZF2 (CX4CXnHXnX4H), ZF3 (CX2CXnHX3C) and ZF4 (CXCXnHX3C). Quantitative real-time PCR analysis revealed that two genes expressed in different organs, and showed a dynamic expression pattern in Populus adventitious roots. Transient gene expression assay in Populus mesophyll protoplasts demonstrated that PeMGP and PeJDK were local-ized in nucleus, and their protein interact each other in nucleus.