CAJ | 학술논문

克隆了1条白桦MYB转录因子基因，命名为BpMYB2。实时定量PCR分析表明BpMYB2基因在白桦不同器官和组织内的表达量不同，在直立木木质部中表达量最高，其次是人工弯曲处理2周的对应木、应拉木、花序，在叶和芽中表达量极低；利用染色体步移技术，获得长度为1284 bp的启动子序列。启动子进行PLACE分析发现，序列中含有TATA box、ARR1AT、WRKY71OS等元件。构建了由BpMYB2启动子驱动GUS报告基因在植物中的表达载体，命名为BpMYB2-1301，利用农杆菌介导法瞬时转化白桦幼苗，并进行组织化学染色。结果表明：BpMYB2的启动子具有启动子活性，能够驱动GUS基因在白桦中表达；GUS瞬时表达信号在茎、叶柄中较强，在叶片中较弱，说明BpMYB2基因与白桦木质部发育相关。
극륭료1조백화MYB전록인자기인，명명위BpMYB2。실시정량PCR분석표명BpMYB2기인재백화불동기관화조직내적표체량불동，재직립목목질부중표체량최고，기차시인공만곡처리2주적대응목、응랍목、화서，재협화아중표체량겁저；이용염색체보이기술，획득장도위1284 bp적계동자서렬。계동자진행PLACE분석발현，서렬중함유TATA box、ARR1AT、WRKY71OS등원건。구건료유BpMYB2계동자구동GUS보고기인재식물중적표체재체，명명위BpMYB2-1301，이용농간균개도법순시전화백화유묘，병진행조직화학염색。결과표명：BpMYB2적계동자구유계동자활성，능구구동GUS기인재백화중표체；GUS순시표체신호재경、협병중교강，재협편중교약，설명BpMYB2기인여백화목질부발육상관。
In this study, a MYB transcription factor named BpMYB2 was cloned from Betula platyphylla. The realtime PCR analysis showed that the expression of BpMYB2 was various in different tissues, and it expressed in the highest level in normal wood, then the second level in opposite wood, and then in tension wood and flower, while the least expression were in leaves and buds. A 1 284 bp BpMYB2 promoter was cloned by genome walking method, and the promoter includ-ed some cis-elements such as TATA box, ARR1AT, WRKY71OS by PLACE analysis. Further, the BpMYB2 promoter was inserted to pCAMBIA1301 vector under control of the 35S promoter to generate the BpMYB2-1301 recombinant con-struct, which was transient expressed in B. platyphylla seedlings by Agrobacterium tumefaciens mediated method. The histochemical GUS staining showed that the BpMYB2 promoter could drive the expression of GUS gene in B. platyphylla, and expressed in high level in stems and petioles while in low level in leaves. In conclusion, BpMYB2 is important forxy-lem development and lay basis for utilizing the transcription factor to regulate the xylem development in B. platyphylla.