CAJ | 학술논문

目的：探讨泽兰水煎剂对实验性小鼠溃疡性结肠炎急性期结肠黏膜病理的影响。方法健康雄性昆明小鼠60只，按随机数字表法分为对照组、模型组和泽兰组，每组20只。对照组自由饮用蒸馏水＋生理盐水灌胃，模型组饮用含5％葡聚硫酸钠（ DSS ）的蒸馏水＋生理盐水灌胃，泽兰组饮用含5％ DSS的蒸馏水＋泽兰水煎剂灌胃。实验结束时处死小鼠，取近肛门2 cm处结肠组织，制作病理组织切片，光镜下观察。结果模型组、泽兰组均成功建立小鼠溃疡性结肠炎急性期模型。实验结束时对照组、泽兰组小鼠体重均明显大于模型组（P＜0．05），但对照组小鼠体重与泽兰组比较，差异无统计学意义（P＞0．05）。实验结束时对照组、泽兰组小鼠体重均明显高于实验前（P＜0．05），实验结束时模型组小鼠体重明显低于实验前（P＜0．05）。对照组和泽兰组小鼠结肠标本大体形态和组织形态学评分均明显低于模型组（P＜0．05）；而泽兰组结肠标本大体形态和组织形态学评分与对照组比较，差异均无统计学意义（ P＞0．05）。结论泽兰能明显减轻DSS所致小鼠溃疡性结肠炎的急性期病理改变。
목적：탐토택란수전제대실험성소서궤양성결장염급성기결장점막병리적영향。방법건강웅성곤명소서60지，안수궤수자표법분위대조조、모형조화택란조，매조20지。대조조자유음용증류수＋생리염수관위，모형조음용함5％포취류산납（ DSS ）적증류수＋생리염수관위，택란조음용함5％ DSS적증류수＋택란수전제관위。실험결속시처사소서，취근항문2 cm처결장조직，제작병리조직절편，광경하관찰。결과모형조、택란조균성공건립소서궤양성결장염급성기모형。실험결속시대조조、택란조소서체중균명현대우모형조（P＜0．05），단대조조소서체중여택란조비교，차이무통계학의의（P＞0．05）。실험결속시대조조、택란조소서체중균명현고우실험전（P＜0．05），실험결속시모형조소서체중명현저우실험전（P＜0．05）。대조조화택란조소서결장표본대체형태화조직형태학평분균명현저우모형조（P＜0．05）；이택란조결장표본대체형태화조직형태학평분여대조조비교，차이균무통계학의의（ P＞0．05）。결론택란능명현감경DSS소치소서궤양성결장염적급성기병리개변。
Objective To investigate the effect of lycopus decoction on the pathological changes of colonic mucous membrane in mice with ulcerative colitis (UC) in acute phase.Methods Sixty healthy male Kunming mice were divided into control group ,model group and lycopus group according to the random number table ,with 20 mice in each group .The mice in the control group freely drank distilled water and were gavaged with normal saline;In the model group,instead of 5%dextran sulfate sodium(DSS) solution plus normal saline by gavage;In the lycopus group, replaced by 5%DSS solution plus lycopus decoction by gavage .The animals were killed at the end of experiment ,and then the colon tissue sampling which was 2 cm near anus was performed , and then the pathological sections were observed with light microscope .Results The mouse model of ulcerative colitis in acute phase was established successfully both in the model group and lycopus group .At the end of experiment ,the body weight of mice in the control group or lycopus group was heavier than that in the model group (P<0.05),but there was no significant difference in the body weight of mice between control group and lycopus group (P>0.05).The body weights of mice in the control group and lycopus group at the end of experiment were heavier than those before experiment ( P<0.05) ,the body weight of mice in the model group at the end of experiment was lighter than that before experiment (P<0.05).The scores of gross morphology and histomorphology of colonic samples in the control group and lycopus group were significantly lower than those in the model group (P<0.05).There were no significant differences in the scores of gross morphology and histomorphology of colonic samples between lycopus group and control group ( P>0.05).Conclusion Lycopus can significantly alleviate the acute pathological changes of colonic mucosa membrane in mice with DSS -induced UC .