海洋科学
海洋科學
해양과학
MARINE SCIENCES
2014年
2期
76-79
,共4页
邹琰%李莉%张良%吕芳%辛美丽%张少春%邱兆星%刘洪军
鄒琰%李莉%張良%呂芳%辛美麗%張少春%邱兆星%劉洪軍
추염%리리%장량%려방%신미려%장소춘%구조성%류홍군
菲律宾蛤仔(Ruditapes philippinarum)%地理群体%遗传多样性%AFLP
菲律賓蛤仔(Ruditapes philippinarum)%地理群體%遺傳多樣性%AFLP
비률빈합자(Ruditapes philippinarum)%지리군체%유전다양성%AFLP
Ruditapes philippinarum%geographical populations%genetic diversity%AFLP
利用AFLP技术对山东沿海菲律宾蛤仔(Ruditapes philippinarum)4个野生群体(乳山、无棣、牟平、即墨)进行了遗传多样性分析。采用6对引物组合对4个群体进行扩增,共得到356个位点,乳山群体、无棣群体、牟平群体和即墨群体的多态位点比例依次为74.40%、70.50%、72.80%、74.70%,具有较高的遗传多样性水平。其中,即墨群体多态性比例最高,无棣群体多态性比例最低。聚类分析表明,各群体间的遗传距离为0.0263~0.0448,乳山群体和无棣群体间的遗传距离最近(0.0263),两群体首先聚在一起,随后与牟平群体和即墨群体聚在一起。
利用AFLP技術對山東沿海菲律賓蛤仔(Ruditapes philippinarum)4箇野生群體(乳山、無棣、牟平、即墨)進行瞭遺傳多樣性分析。採用6對引物組閤對4箇群體進行擴增,共得到356箇位點,乳山群體、無棣群體、牟平群體和即墨群體的多態位點比例依次為74.40%、70.50%、72.80%、74.70%,具有較高的遺傳多樣性水平。其中,即墨群體多態性比例最高,無棣群體多態性比例最低。聚類分析錶明,各群體間的遺傳距離為0.0263~0.0448,乳山群體和無棣群體間的遺傳距離最近(0.0263),兩群體首先聚在一起,隨後與牟平群體和即墨群體聚在一起。
이용AFLP기술대산동연해비률빈합자(Ruditapes philippinarum)4개야생군체(유산、무체、모평、즉묵)진행료유전다양성분석。채용6대인물조합대4개군체진행확증,공득도356개위점,유산군체、무체군체、모평군체화즉묵군체적다태위점비례의차위74.40%、70.50%、72.80%、74.70%,구유교고적유전다양성수평。기중,즉묵군체다태성비례최고,무체군체다태성비례최저。취류분석표명,각군체간적유전거리위0.0263~0.0448,유산군체화무체군체간적유전거리최근(0.0263),량군체수선취재일기,수후여모평군체화즉묵군체취재일기。
The AFLP technique was applied to investigate genetic diversity of 4 geographical populations of Rudi-tapes philippinarum in coastal sea of Shandong. Six primer pairs were used to generate 356 loci among individuals in 4 populations. The proportion of polymorphic loci for the Rushan, Wudi, Muping and Jimo populaton was 74.40%, 70.50%, 72.80%and 74.70%respectively, suggesting high levels of genetic diversity. The Jimo population exhibited the highest genetic diversity, and the Wudi population exhibted the lowest one. The cluster analysis showed that the genetic distance among the 4 populations was 0.0263~0.0448 and the genetic distance between the Rushan and Wudi population was the smallest one (0.0263). The Rushan and Wudi populations were clustered to-gether and subsequently grouped with Muping-Jimo clade.