CAJ | 학술논문

为获得琼胶酶活性高产菌株,对采集的海水样品进行了产酶微生物的分离、筛选和鉴定。经过平板培养预筛和两次摇瓶培养筛选,从浙江省舟山市普陀区近海海水样品中分离到一株琼胶酶产生菌 G-5,该菌株液体培养产酶活力可达到413.8 U/mL。G-5菌株的菌落呈乳白色、半透明、表面湿润；菌体革兰氏染色阴性,大小0.58~0.69μm×1.50~2.26μm；光学显微镜下呈短杆状,略有弧形。G-5菌株的16S rDNA序列与NCBI数据库中15个弧菌属(Vibrio)菌株的16S rDNA有98%的同源性,可以确定G-5菌株是一株弧菌(Vibrio sp.)。
위획득경효매활성고산균주,대채집적해수양품진행료산매미생물적분리、사선화감정。경과평판배양예사화량차요병배양사선,종절강성주산시보타구근해해수양품중분리도일주경효매산생균 G-5,해균주액체배양산매활력가체도413.8 U/mL。G-5균주적균락정유백색、반투명、표면습윤；균체혁란씨염색음성,대소0.58~0.69μm×1.50~2.26μm；광학현미경하정단간상,략유호형。G-5균주적16S rDNA서렬여NCBI수거고중15개호균속(Vibrio)균주적16S rDNA유98%적동원성,가이학정G-5균주시일주호균(Vibrio sp.)。
To obtain microbes for producing high activity agarase, a series of bacterial strains in sea water were isolated, screened and identified. By prescreening in plate cultivation and twice screening in liquid cultivation, a strain designated as G-5 was isolated from sea water that was taken from Zhoushan coastal areas in Zhejiang Prov-ince. The activity of agarase produced by G-5 could reach 413.8 U/mL. The colony of G-5 was milky white and semitransparent with a wet surface. The cell of G-5 was Gram negative and rod-shaped with a size of 0.58~0.69μm×1.50~2.26μm. The 16S rDNA sequence of G-5 was 98%homologous to 15 strains of Vibrio species in the da-tabase of NCBI. It could be concluded that the strain G-5 was a Vibrio species.