CAJ | 학술논문

构建了一种以海藻酸钠-石墨烯（SA-GR）为基底的新型过氧化氢电化学酶传感器。利用滴涂法将生物相容性良好的海藻酸钠-石墨烯复合物固定在玻碳电极表面，再通过酰胺键将HRP连接在SA-GR复合膜上，从而制备出了性能良好的过氧化氢电化学酶传感器。该传感器重现性好、灵敏度较高，并且响应速度快（3 s），米氏常数较低（Km=0.663），对H2O2检测的线性范围为1.0×10-4～1.2×10-3 mol/L，检测下限为5.7×10-6 mol/L。
구건료일충이해조산납-석묵희（SA-GR）위기저적신형과양화경전화학매전감기。이용적도법장생물상용성량호적해조산납-석묵희복합물고정재파탄전겁표면，재통과선알건장HRP련접재SA-GR복합막상，종이제비출료성능량호적과양화경전화학매전감기。해전감기중현성호、령민도교고，병차향응속도쾌（3 s），미씨상수교저（Km=0.663），대H2O2검측적선성범위위1.0×10-4～1.2×10-3 mol/L，검측하한위5.7×10-6 mol/L。
A new type of enzyme electrochemical sensor for hydrogen peroxide was constructed alginate-graphene (SA-GR) composite. The biocompatible alginate-graphene composite is fixed to the glassy carbon electrode surface by drop-coating method, and then HRP is linked to the SA-GR membrane through an amide bond, thereby the HRP electrochemical biosensor with good performance is prepared for hydrogen peroxide. This biosensor has high sensi-tivity, good reproducibility, fast response (3 s), low Michaelis constant (Km=0.663) and a good linear relationship for detection H2O2 in the range from 1.0×10-4 to 1.2×10-3 mol/L. The detection limit was estimated to be 5.7×10-6 mol/L.