CAJ | 학술논문

目的：通过研究趋化因子Fractalkine（FKN）对人外周血单个核细胞IL-8表达的影响，以及信号分子细胞外调节蛋白激酶（extracellular regulated protein kinases，ERK）、磷脂酰肌醇-3激酶（phosphatidylinositol 3-kinase， PI3K）、核因子-κB（nuclear factor-κB，NF-κB）在其中的作用，探讨FKN促进动脉粥样硬化（atherosclerosis，AS）的机制。方法利用密度梯度离心法分离健康人外周血单个核细胞；将提取的单个核细胞分为：空白对照组、FKN组、PD98059（ERK阻断剂）组、LY294002（PI3K阻断剂）组、PDTC（NF-κB阻断剂）组、FKN＋PD98059组、FKN＋LY294002组、FKN＋PDTC组；分别于培养12 h和24 h后收集细胞培养上清，应用ELISA检测各组单个核细胞中IL-8的表达情况。结果（1）细胞培养12 h或24 h后，PD98059组、LY294002组和PDTC组与空白组比较，IL-8表达有减少趋势，但差异无显著性意义（P＞0．05）。（2）FKN组较空白组IL-8表达明显减少（P＜0．05），FKN＋PD98059组和FKN＋LY294002组较FKN组IL-8表达明显增加（P＜0．05），FKN＋PDTC组较FKN组、FKN＋PD98059组和FKN＋LY294002组IL-8表达均明显减少（P＜0．05）。（3）细胞培养24 h后，各组IL-8表达均较12 h明显减少（P＜0．05）。结论趋化因子FKN可能通过ERK、PI3K信号途径抑制单个核细胞IL-8的表达，而通过NF-κB途径促进IL-8的表达，FKN对单个核细胞IL-8的表达具有双向调节作用，但以抑制作用为主。
목적：통과연구추화인자Fractalkine（FKN）대인외주혈단개핵세포IL-8표체적영향，이급신호분자세포외조절단백격매（extracellular regulated protein kinases，ERK）、린지선기순-3격매（phosphatidylinositol 3-kinase， PI3K）、핵인자-κB（nuclear factor-κB，NF-κB）재기중적작용，탐토FKN촉진동맥죽양경화（atherosclerosis，AS）적궤제。방법이용밀도제도리심법분리건강인외주혈단개핵세포；장제취적단개핵세포분위：공백대조조、FKN조、PD98059（ERK조단제）조、LY294002（PI3K조단제）조、PDTC（NF-κB조단제）조、FKN＋PD98059조、FKN＋LY294002조、FKN＋PDTC조；분별우배양12 h화24 h후수집세포배양상청，응용ELISA검측각조단개핵세포중IL-8적표체정황。결과（1）세포배양12 h혹24 h후，PD98059조、LY294002조화PDTC조여공백조비교，IL-8표체유감소추세，단차이무현저성의의（P＞0．05）。（2）FKN조교공백조IL-8표체명현감소（P＜0．05），FKN＋PD98059조화FKN＋LY294002조교FKN조IL-8표체명현증가（P＜0．05），FKN＋PDTC조교FKN조、FKN＋PD98059조화FKN＋LY294002조IL-8표체균명현감소（P＜0．05）。（3）세포배양24 h후，각조IL-8표체균교12 h명현감소（P＜0．05）。결론추화인자FKN가능통과ERK、PI3K신호도경억제단개핵세포IL-8적표체，이통과NF-κB도경촉진IL-8적표체，FKN대단개핵세포IL-8적표체구유쌍향조절작용，단이억제작용위주。
Objective To observe the effect of chemotatic factor FKN on IL -8 expression of monocytes in peripheral blood and the function of extracellular regulated protein kinases ( ERK), phosphatidylinositol 3 -kinase ( PI3K) and nuclear factor-κB( NF-κB) .Methods Peripheral blood monocytes were isolated from fresh blood of healthy volunteers by the den -sity gradient centrifugation.The isolated monocytes were divided into eight groups:control, FKN, PD98059, LY294002, PDTC ( NF-κB inhibitor ) , FKN+PD98059 , FKN+LY294002 , FKN+PDTC.At 12 h and 24 h, respectively , the su-pernatants of monocytes were collected from each group ,and the IL-8 expressions of monocytes in every group were detec-ted with ELISA.Results The expressions of IL-8 in PD98059, LY294002 and PDTC group showed a decreasing trend , but not statistically significant (P>0.05),compared with that of the control and the expressions of IL -8 in FKN group was decreased compared with that of control (P <0.05).The expression of IL -8 from FKN +PD98059 and FKN +LY294002 group were increased compared with that from FKN group (P<0.05).The expression of IL -8 from FKN+PDTC was significantly decreased compared with those from FKN、FKN+PD98059 and FKN +LY294002 (P<0.05). Cells cultured for 24 h group,IL-8 expression in each group was significantly decreased compared with 12 h (P<0.05). Conclusion Chemokine factor FKN could inhibit IL -8 expression in mononuclear cells through the ERK、PI3K pathway, promote the expression of IL -8 by NF-κB pathway .