心血管康复医学杂志
心血管康複醫學雜誌
심혈관강복의학잡지
JOURNAL OF CARDIOVASCULAR REHABILITATION MEDICINE
2014年
2期
113-116
,共4页
孙玮%蔡尚郎%宋宇%张继东
孫瑋%蔡尚郎%宋宇%張繼東
손위%채상랑%송우%장계동
牙周疾病%动脉硬化%细胞粘附分子%基质金属蛋白酶类
牙週疾病%動脈硬化%細胞粘附分子%基質金屬蛋白酶類
아주질병%동맥경화%세포점부분자%기질금속단백매류
Periodontal diseases%Arteriosclerosis%Cell adhesion molecules%Matrix metalloproteinases Article number 1008-0074 (2014)02-113-05
目的：观察牙龈卟啉单胞菌（Pg，为牙周病的主要致病菌）对血管内膜粘附因子和金属蛋白酶的影响。方法：大鼠120只，按数字表随机分为空白对照组、Pg低剂量组（2ml）与 Pg高剂量组（5ml），各40只，后两组每3 d分别接受相应剂量 Pg肌注，且均不进行抗生素干预，历时12周。取其静脉血，检测三组血液中的细胞间粘附分子-1（ICAM-1）、血管细胞粘附分子-1（VCAM-1）、基质金属蛋白酶（MMP）-3及 MMP-9的水平。结果：与空白对照组相比，Pg低剂量组与高剂量组大鼠静脉血中 ICAM-1[（175.79±14.30）ng/ml比（182.62±15.07） ng/ml、（189.39±14.93）ng/ml]、VCAM-1[（256.49±37.17）ng/ml 比（271.58±32.85）ng/ml、（286.66±30.66）ng/ml]、MMP-3[（3.23±0.69）ng/ml 比（3.61±0.82）ng/ml、（3.97±0.83）ng/ml]及 MMP-9[（1.30±0.39）mg/L比（1.48±0.39）mg/L、（1.67±0.45）mg/L ]含量显著增加（P＜0.05或＜0.01），且与Pg低剂量组相比，Pg高剂量组上述因子含量显著增加（P＜0.05）。结论：牙龈卟啉单胞菌可引起大鼠血清中血管内膜粘附因子和金属蛋白酶含量的显著增加，可加剧冠心病的病理演变。
目的：觀察牙齦卟啉單胞菌（Pg，為牙週病的主要緻病菌）對血管內膜粘附因子和金屬蛋白酶的影響。方法：大鼠120隻，按數字錶隨機分為空白對照組、Pg低劑量組（2ml）與 Pg高劑量組（5ml），各40隻，後兩組每3 d分彆接受相應劑量 Pg肌註，且均不進行抗生素榦預，歷時12週。取其靜脈血，檢測三組血液中的細胞間粘附分子-1（ICAM-1）、血管細胞粘附分子-1（VCAM-1）、基質金屬蛋白酶（MMP）-3及 MMP-9的水平。結果：與空白對照組相比，Pg低劑量組與高劑量組大鼠靜脈血中 ICAM-1[（175.79±14.30）ng/ml比（182.62±15.07） ng/ml、（189.39±14.93）ng/ml]、VCAM-1[（256.49±37.17）ng/ml 比（271.58±32.85）ng/ml、（286.66±30.66）ng/ml]、MMP-3[（3.23±0.69）ng/ml 比（3.61±0.82）ng/ml、（3.97±0.83）ng/ml]及 MMP-9[（1.30±0.39）mg/L比（1.48±0.39）mg/L、（1.67±0.45）mg/L ]含量顯著增加（P＜0.05或＜0.01），且與Pg低劑量組相比，Pg高劑量組上述因子含量顯著增加（P＜0.05）。結論：牙齦卟啉單胞菌可引起大鼠血清中血管內膜粘附因子和金屬蛋白酶含量的顯著增加，可加劇冠心病的病理縯變。
목적：관찰아간계람단포균（Pg，위아주병적주요치병균）대혈관내막점부인자화금속단백매적영향。방법：대서120지，안수자표수궤분위공백대조조、Pg저제량조（2ml）여 Pg고제량조（5ml），각40지，후량조매3 d분별접수상응제량 Pg기주，차균불진행항생소간예，력시12주。취기정맥혈，검측삼조혈액중적세포간점부분자-1（ICAM-1）、혈관세포점부분자-1（VCAM-1）、기질금속단백매（MMP）-3급 MMP-9적수평。결과：여공백대조조상비，Pg저제량조여고제량조대서정맥혈중 ICAM-1[（175.79±14.30）ng/ml비（182.62±15.07） ng/ml、（189.39±14.93）ng/ml]、VCAM-1[（256.49±37.17）ng/ml 비（271.58±32.85）ng/ml、（286.66±30.66）ng/ml]、MMP-3[（3.23±0.69）ng/ml 비（3.61±0.82）ng/ml、（3.97±0.83）ng/ml]급 MMP-9[（1.30±0.39）mg/L비（1.48±0.39）mg/L、（1.67±0.45）mg/L ]함량현저증가（P＜0.05혹＜0.01），차여Pg저제량조상비，Pg고제량조상술인자함량현저증가（P＜0.05）。결론：아간계람단포균가인기대서혈청중혈관내막점부인자화금속단백매함량적현저증가，가가극관심병적병리연변。
Objective:To observe influence of porphyromonas gingivalis (Pg,a main pathogenic bacterium of perio-dontal disease)on vascular intima adhesion factors and metalloproteinases.Methods:A total of 120 rats Were ran-domly and equally divided into blank control group,Pg loW dose group (Pg 2ml)and Pg high dose group (Pg 5ml) according to number table.The latter tWo groups respectively received intramuscular injection of corresponding dose Pg every three days Without antibiotic intervention for 1 2 Weeks.Then their venous blood Was taken to measure lev-els of intercellular adhesion molecule-1 (ICAM-1 ),vascular cell adhesion molecule-1 (VCAM-1 ),matrix metallo-proteinase (MMP)-3 and MMP-9 in three groups.Results:Compared With blank control group,there Were sig-nificant increase in contents of ICAM-1 [(175.79±14.30)ng/ml vs.(182.62±15.07)ng/ml,(189.39±14.93) ng/ml]、VCAM-1 [(256.49±37.17)ng/ml vs.(271.58±32.85)ng/ml ,(286.66±30.66)ng/ml]、MMP-3 [(3.23±0.69)ng/ml vs.(3.61±0.82)ng/ml,(3.97±0.83)ng/ml]and MMP-9 [(1.30±0.39)mg/L vs. (1.48±0.39)mg/L,1.67±0.45)mg/L](P<0.05,or <0.01),Compared With Pg loW dose group,there Were significant increase in levels of above indexes in Pg high dose group (P<0.0 5 ).Conclusion:Porphyromonas gingi-valis can significantly increase serum contents of vascular intima adhesion factors and metalloproteinases,aggravat-ing pathological development of coronary heart disease.
