CAJ | 학술논문

目的：研究不同形式的HIV DNA与疾病进展之间的关系。方法采用实时定量PCR法来检测48例具有明确感染时间的患者总的、整合的及2个长末端重复序列（long terminal repeat, LTR）的环状HIV DNA。分析随着时间的变化，不同形式HIV DNA的动力学变化特点及其与疾病进展的关系。结果随着疾病的进展，总的及整合的HIV DNA增加。总的HIV DNA与病毒载量呈正相关。总的及整合的HIV DNA与CD4+T淋巴细胞计数呈负相关。2个LTR的环状HIV DNA与病毒载量及CD4+T淋巴细胞计数无相关性。结论总的及整合的HIV DNA与疾病进展密切相关，2个LTR的环状HIV DNA与疾病进展无相关性。
목적：연구불동형식적HIV DNA여질병진전지간적관계。방법채용실시정량PCR법래검측48례구유명학감염시간적환자총적、정합적급2개장말단중복서렬（long terminal repeat, LTR）적배상HIV DNA。분석수착시간적변화，불동형식HIV DNA적동역학변화특점급기여질병진전적관계。결과수착질병적진전，총적급정합적HIV DNA증가。총적HIV DNA여병독재량정정상관。총적급정합적HIV DNA여CD4+T림파세포계수정부상관。2개LTR적배상HIV DNA여병독재량급CD4+T림파세포계수무상관성。결론총적급정합적HIV DNA여질병진전밀절상관，2개LTR적배상HIV DNA여질병진전무상관성。
Objective To investigate the correlation between different forms of HIV DNA and disease progression. Methods Re-altime quantitative method was used to detect total and integrated HIV DNA and HIV 2-long terminal repeat (LTR) circular DNA in peripheral blood of 48 patients with definite infection time. With time went on, dynamic features of different forms of HIV DNA and the correlation between different forms of HIV DNA and disease progression were analyzed. Results With the progression of the disease, the levels of total and integrated HIV DNA increased. Total HIV DNA was positively correlated with viral load. Total and integrated HIV DNA was negatively correlated with CD4+T lymphocyte count. There were no correlation between HIV 2-LTR cir-cular DNA and viral load and CD4+T lymphocyte count. Conclusions Our results suggest that it is total and integrated HIV DNA, rather than HIV 2-LTR circular DNA that are closely correlated with disease progression.